Quick Start

cncRNADetector is an accurate bioinformatics pipeline for identification of coding and noncoding RNA such as lncRNA , miRNA and other through combining all the filtering steps into a single platform. It discriminate between non-coding and protein-coding transcripts. Futher in non-coding, it identify miRNA and lncrna. It takes the assembled transcript sequences in FASTA format as input and gives the output of every step in separate files.

Pre-Requisite

• BLAST
• RNAfold
• Ubuntu/Cygwin
• Databases (pataa, swissprot, mature miRNA , tRNA, rRNA)
• Perl

RUN

Default code example:

cncRNADetector.pl -h

cncRNADetector.pl -m <full|assembly|lncRNA|miRNA> -i <filename>

miRNA.pl -i <filename>

There are two ways to submit RNA transcript sequences:

• Paste RNA sequences in fasta format into the input text box at the homepage.
• Upload fasta file of the sequences need to be identified using the upload button at the homepage

User shall have the ability to view processing step of cncRNADetector.

• User can track either by submitting task id or mail id on result page.

• By clicking on track steps view cncRNADetector processing..

After finishing identification process, results will be shown on browser. Also, cncRNADetector will assign an unique Task ID for each jobrequest.

• Users can also retrieve results by either email-id or by inputting the Task ID in the result.
• You can also download the result table file in tab delimetered file(.txt,.csv etc) format.