lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-273.88) is given below.

1. Sequence-
ACCACUUUCUUAUAUUUCAACUAUUUCUUCAAAAUCAACAAACAACCCCACAUACCAAGU
UUUUAUCUUUUAGCUAUUUCUUCCAGAAAAAACCACCACCCCUUUUGCAUCUGAUAAAAA
ACAACAAAAUCCAGUACAGAAAAUGUCUACACAUUUCAAGUAAACAACAUAUAUAACUCG
AUGUGGUUGCUUCCAUCUGUUCUAGCCUUGUGGACAGAUUUAUCUCAUACCUACUGCCCG
UGGGAAGUGACAGAUAUCCCGUGCAAUUAGUCAAGCUACGUGAAAACUAGCCUGGACACC
ACGGUUAUCAAAAAACAAAACAACAUAUAUAACUUGAUUCGAUAAAUCAUCAAUUCCAAU
UUGAAAUUCGAAACCAAGUUCAAUCUUCAAAAAAAUUUCCCCAAACCAACAACAAUGUAC
CCAUUUUAAUCCCACAAGUGGGGUCUAGGGUGUACGAAGACGUUAUCCCUACCUAGUGCG
AGACCCUUGACUCAAGUAAAAAUAUGUACAAAGCAACUGGAAAAAGGAAAACAAAAGGAG
AUCAUUUUUUUCCUCAACCAGAAACCACUUUCUUAUAUUUCAACUAUUUCUUCAAAAUCA
ACAAACAAACCCACAUACCAAGUUUUUAUCUUUUAGCUAUUUCUUCCAAAAAAAACAACA
ACCCUUUUACAUCUGAUCAAAAACAACAAAACCCAAUACAGAAAAUGUCUAUACAUUUCA
AGUAAACAACAUAUAUAACUCUGGUGUUGUUUCUUCCCAUCUUGGUGGAUAGAGUUACGU
GAUACUAUUGUUAGUGGGAGGUGAAAAGUAUCCUUGAUUAAUAAUCAAGGUGCGCGAAAG
CUAACCCGGACUCCACGGUUAUAAAAAACAACAUCUAUAACUCGAUUCAAUCAAUCAUCA
AUUCCAAUUUGAAAUUUGAAACCAAGUUCAAUCUACAAAUUCUAUCCCCAAAUCAACAAC
AAACACACUGUGAUCCCACAAUGGUAUCUGCGGAGGAUAUUGUGUACCCUUACCUACCAC
UACCUUGUAAUAUAGACCCUCGACUCAACUCAAGUAAAAACAUUACAAAGUAAUUCGAAA
AAGGAAAACAAACAUGAGAUCUUCCACUUUUUCCACAAUCAGAAAUCAAUCUCUUAAAUU
UCCUCUAUUUCUUCAAAAUCAACAAAUCCCACAUACCAAGUUUCUGUCUUUCUGCUAUUU
CUUCCAUAAAAACAUCCACCCUCUUUGCAUCUAAUCAAAACUAACCAAAACCCAGUUCAG
AAACACCUCAAUAUUUCAAGAAACCAACAAAUAUAAUGUUUCUUGAAACCGUAAAAAUUC
UAGUUUUCAAGAUCAAUACAUAAGUAAUAUAUGUAAAGGCAAAUCCAAUACAAACUACGA
AAAGGGUAUUUGAAAUUGAGGGAAAUAAAAUCAACAAACCAGUAAAUUUAGCAAAAAUCG
UAUGACAGAUCCAAUGGAGAACUAAAAAAUUCUUGGAAAACUUUCAACUUUUCAACGCAC
ACGCUAUAUAGAUAUGUAAUAAAUUUUGGAAAAUUAGGAGAGAUUGAGAUAAUUAAUUUG
GAGUUGGACUCGGAAUUGGUGUUGUUCACACUUCCAAAUCACAUAAAUUCCAGAAAAAAU
CAGUCUGGCAAUUCCUCCUCCAUUCCUUUCCUUUUUUACUUUUCU

2. MFE structure-
.......(((((.(((((..(((.((.((((((((...................(((((..(((....(((((....((((.(((............((...((((((.((((((.................((.(((.....))))).((((((...(((..............))).))))))(((((((..((((((
((.........)))))))).....((((.(((((.....)))))..))))..((((..(((((.......(((.((((.((....)))))).)))....))))).)))).............((((((...(((((((((((....(((............)))...)))))...(((......))).............
........(((....))).(((((((.........)))))))(((.(((((((((....................))))...))))).)))))))))....))))))...)))))))(((((((((.........((((((.......)))))).....(((((....)))))...........................
...........((.((((((.(((((((((............................................))).))))))..................((((((....)))))).(((.(((((((((...........))))))))))))......((((.((((((((((....(((((((....))))(((.(
((.....(((.((((((((...)))))))))))......)))..)))(((((...((((((((............)))))).))......((((...((((.......))))...)))).....))))).)))....)))))))))))))).................((((....)))).)))))).)).(((((((.(
(((((..((((.........(((.(((((((.......((.((......)).)).......))))))).)))........)))).......)))))).))))))).)))))))))..))))))...............(((((((.(((((............(((((((.........(((((((.....(((......
....................................................)))..))))))).........(((((((((((.............))))))))))).........(((((((((..........((((((.......))))))..((.....)).....)))))).)))..))).))))))))).)))
))))...((((..((......))..)))).))))))...)).............)))))))))))))))..)))))....................((....))(((((....)))))....)))))))).)).)))..))))))))))..........(((((.(((...(((((((((((.....)))).........
.........(((((.........))))))))))))...))))))))...................

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-303.06] kcal/mol.

2. The frequency of mfe structure in ensemble 2.74645e-21.

3. The ensemble diversity 396.27.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS