lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-440.60) is given below.

1. Sequence-
GGGGGGGGGUUCUCUGCCCUUCAAGGCUAUCAUUUAGGAGGGUAAGCAAGGACCUGUCAA
GUCACAGAUAACAGACUGAUCUAUGUUACUGAUUGACAUGAUAAAAUUCCUAAACAAGAU
GCUAAUGAAAGCUAGUGUAAAUUGCAGUCAAAAGCAAAACACAAACCUGAAGCAGAGACU
CACAAGGAACAAUAUUAGAGUACGAAUCUUUACUCUUUCAGGAUAGACAACAUGGUCAAA
GAACUUCAAACAGAAAUCCAAAGAAAAUGAGAUAACAGAGCCAGGAAUUAAGAUAAAAGC
AAGCAAUUGUAAAACUCCCUUUUAUCCUUAAAAGAAGGAUGUAACUGAGGUACAAACUCC
UUCAGAAAGGUUUUCUGAUGAAUCUUAGUAUAGAAAAUAUGUAGUCAUAGAAGGUUCAUG
CCCUAAACGUACCCCAAACAAGAAUACACCAUUUCAAACAAAGGACUUCAAUGAAGACAC
AAAGUGAGGCCUGCCACAACCAGGUCAUAAGCAGGAAGCCAACAAAAACACAACAUAACU
GACCAACCAAAUUGGGCAAACUACAAAGCUCCUGUCUUCCAACCAAGCUAUAUACUUCUA
AAAAUAAUAGUCAAAGAAGCUUGCAAUCAGCCUAACAAACCUCUAUUUAUCUCAGCCUAG
ACACACAUACUAGGGGGAUUUGACAUUAUACAACAAUAACACCCUAUGACUCUGAUAAAU
AAAGAAAACUUUUCAAUUAUGCAACAAUCAAGUCUAAAUAACCAAGAAGAUACAAAAAUC
CUAACUUACCAUGAAGAAUAAGGACUUAAAAAAUAGAUCCCGAACCUGUUAUUAUGAAAU
GAAUUUAACACAGAAAGGCUCCAAAACUCCACCUCAAGGUAUAAAACAAGCAUGUCAAGU
GAAAUAAUCUAUUAGGUUGAUCAUACAAGCAUGCUUGAACUCUACAGCACCAACAACAAA
AAAUAGCUCCUCUAUCCUAGACACUAGAGGGCCUAAGCAUGUAGUAGAUAGUAUAACACA
AGAGGAUAACCUAUUUUUUUAGGAAAAUGGGAUCUCAUAGAUUGCAUGAGAUUUGGAUCA
AAGUGAACCAAAAACUCAUAUUUCAUCUUAAACAUGAAGCUCAGGUACACAAACAACAUG
AAUGGUUCACAUCUUUGCCCAGAAAUAUCUAUCCACACACACUACUUUUAAUCAAAAGAA
AACAAAUACCUCCUUAUUUAUUCAAACACUUAAAACAAAUGUAACACAACUCUUUCUGAU
GAUAAAAACAUGCACAAAACUCACAGAAACAUCACUCACAAAAACAUCUUUAUAGACAAA
UAACAAAGUAAUGCAGGGAUUAGAGCAUCUCUCAACUUUUUUGGAGUAGGAAUUUGGGAU
UUCUAGCAAGUUGAGAGUGAGUUUGCUACGUAUACAGCUGUUUUGACUAAAUUUACAAAU
CUUCCUUCCUCGUCUAUGAGAGAAAACUUAUGUUCUGGACACAUACAUUGGACCAGCCGC
CCUUAGAAUCCUCGAUAUCCUAAAUCUAUCAACUAUGAACCUUAAUCGUCUAUUUCUUGU
UUGUGAACCUACGAGAAAACUAAGGAUACUCAUUCUCUCGAACCCUAAGGUUAUUGACUG
CUGAGACUAUCAUUCCCUACGGCUAACCCUAAAUCUCUCUACAACUUUGCUUAUAUAAAA
GAGAACACUAGAGUUUGAGCGGAAAACCCAAUAUAGGUGGAAAAAUCAUGCACCUCAUAG
UACACAGAGCCAAGAUCAACAGUCAAAAAUCAAGAGGAUUUGAAAUUUGAAAACCAAUUC
GUUGGGGGUUUUGGAAUCCACAAAUUAAAGGCAAUAACAAUAUGAAAAAUGGUAAGAACA
ACGUUUUUACAGAUGGAUUUGAAGAGAAACAAUGAGAAUUUACCGAAUAUUACCGUUGGU
GAGAUUUUGAGGCUACAAACUCACCCAUCUCGUGUUGUAAUGACAUUAAACGGGCGAAUA
ACGAGUAUAGAAUCUUAAAUUGUCAAUUUGAUGGAGAAAAGAAGAGAUUUGAGGUUGUUU
GGUAUGAUUGUUGACUGAAACUUGAAGUUAGAUGGAACUAGGGGUGUUUCCGUGUUCUUC
ACUCACCGAAGAGAGAGAGAAUAGAGAAAAAAGAGAGAGACACAGUCAGAGGUAGCGGAG
GGUAUUGAGUCGCGUCUGAAGGAUGCGCGUAUAUUUCAAUCAAGG

2. MFE structure-
(((.((((((.((((((..((((.((..(((.(((((((((((........)))(((((.(((((((.(((((..........))))))))..)))).)))))...))))))))...)))(((......)))..((((...((((........)))).))))...))))))))))))))))..(((((........((((
((........))))))....(((((((.....(((.((((((..(((((((((..............((((((((.((((.(((((.((((((((((((..((...........))..))))))).)))))..((((((((((.......))))...)))))).....((((((((((((((((((..(((.((......
....))))).))))))))).............((.......(((........))).......)).((((...)))).(((...))).((((((.......)))))).....)))))))))...................((.((((.....)))).))...........))))).........((((((........(((
.......))).......)))))).................))))..)))))))).(((((.........)))))((((((((.........................((((.((((..((((.((((...))))..))))......))))))))........................((((..(((......)))....
)))).........))))))))....)))))....))))..................((.((((((.(((..((((...........(((((((((...((((..((((((...)))))).))))....)))))))))..(((((((((...............(((.(((((((..........))))))).))).)).)
)))).))..............))))....((((......))))...(((.(((((...))))).)))))).))))))))...((((((((..........((((((.......)))))).(((.((.........)).))).)))))))).)))))).)))......)))))))..........(((((....)))))..
..........)))))..............((((((((..((((((.......((((((.(((..................)))))))))((..((((((.......(((....)))..............((((..........)))).(((((((((........((((((((...))))))))..)))))))))))))
)).))....)).))))..))))))))...................((((((......))).)))(((....)))((((...((.....))..)))))).)))........(((((((((((....(((((.........(((((...((...((((((((..........)))))))))))))))...(((((((((((.
((((....)))).....(((((.(((((............((....))(((....(((((...................)))))....)))))))).)))))...(((.......)))((((.......(((((((.((((.((...(((((((.....(((((((.(((((...((.(((........))).))...((
((((....((((.(((((((.....(((((((........(((........)))........)))))))....))))))).))))....)))))).....(((((((((..(((...((((((..((((......)))))))))).(((((...((.......(((((((..(((((.(((.((........)))))..)
))))..))))))).....))...)))))....))))))))))))))))).)))))))...))))..)))...))..)))))))))))))))...((((((.......)))))).)))))))).)))............(((...)))...)))))...))))))))))).(((((((...))))))).............
.....

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-479.72] kcal/mol.

2. The frequency of mfe structure in ensemble 2.72201e-28.

3. The ensemble diversity 534.24.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS