lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-409.70) is given below.

1. Sequence-
AGAGGAACGGGUAAUUCUCUCUAUUCCCUACUUCUUUUCCUUCUGCUUUUUCCUUUUCCC
CUUUUAUCAUUUAGAGUUCCAUAUAAUUGUAUUUGUUUUCCCCAUUGUUUCGAUUAUGCC
UGUAUAUUUGAACUGCAAUUUGGGUAUAACGCAGGCUUGCUCAGUACGUCUAUGGUUUUU
GUGCUGUCUUUAUUUUGCAGUUAAAUUGAUUUUGUGAGGUUAUAUUUUAUUGAUAAUUGU
ACUGUUUCUAUUUUAUUUUUUUGAUCGCUUCAUUGUUUUUCCAAUUUGUCUAAUUGAUUU
GCUUAAUUGAGGCCCUUAUUGGCUAUUCUUGUAUUGAAUUUCUCUUUCCAGGUGUUAGUA
GACGUUCUCAAUGAUCAAGUCUCAGUUCUCUCCAACAAUUCUGCACAGAGGUAAACUUGC
CUAUUUGUGCUUUCUUAUUUCAAGUGCUCCUUUUAAAUUUGUUUUUCCCCUUUUUUGGUG
GAAUACUUCACUUAUUUGUUGUUAUCAUGUUGAAUAAUUCACUCUCUUUUCUAUUAGAAU
UAUUUAUAAUUUUGAAAAGAAAGUGGAGGAAUGCAAAAUGAGGGCCUCCAAAGGUCACUC
UAUAAACUUCAAAUGGUUCAUUUACCAUGAGGUAUUAUAUUUUUCUACUUUAAGUUUUAU
UAUUUGCAAGAACAUAUAUUUAAUAUUUUUGUAACUAACAGUUAGUGGUUAUUAAGAGAA
AUCUUGUGAUCAAUGUGAACUAUUUAUAUAUCUAUAGAAAGGAGGGACAUAGUCCCGGUG
AUGUGAUGCUUAAGAGUGAUUAUUUAUCUUUUUUAUUAAUUUUUUACCAUUUUAAAAUUA
AAAAUUCAGUUAUUUACUUAAAUAAAACUCAAUAAUCCCAUAGUUCUUUGGUUAAACAAA
UCCCAGAAGCAGCUACAUUUUUGGAUAAACAAAACUUGACAGUCGCAUCCUGUAUCCACU
UAAAUAUGCCUUCCUUUCUUCCCGUUCGUUCCCCCAUUCUACCCUUUUUACGCCAAAUAC
UCUUCGAAUCAUAAUUGAGUACUUCCAUCUGAGACAUCCUUGAUCCCCUUAUUUAUUUUA
UAAAAAGUGUAACUUGUUUUAGAUUUUAAUCACCUAGGGUCAGUGGCGGGAAUGAACAAC
AAAAAUUCUUGGUUCAGUUGGUCAAGCUAAUCAAUGCAAGGUUAGUUUCCUGUCAGUUCC
AUGUGCAUAUACACAUCUCAACAAUUUGUUCAAUUUUCAUCAAUGUCUCUCGUCAAUCUU
CAAUAAGUAACUCCUUUUCCUUUCUUUUUCUUUCUUCAAAGUAUACGAUACAAUAAUUCC
AGUUGGUUUGUUGCACACAUAGUUAUGGUUCAAUAGGAAAAUGUUUAUUCUUCAAAUUCU
AAAUUUAAAACUUCAUAGGCAUGGAAUGUCAGCUUUUAGUAUUAUUUAUAUAUCUGGAUU
CUGGUUAUAAGGCUGGUGAGUAUAGUCCAAAUGACUUUUGGUUGAUUUAAAGAAAUUUAA
UUACAGUAAGAGAAACAAAUGUUAGUCUUUGCACUUAAAAUUUGUAAACAUAGUUAUAUA
UCGGUUCAUAGUGCCUAUUAUCUCUUGUGCUAGAAGUUGACAGCUUUAGACGUUUUACCA
GUUUAAAGAUAUGAUCAAGAUCGAGAAAAAGUUAACUACUGUUUAAGAAAAAAGGUGGGU
GUAAUGUGUAAAUGUUGAUCGUGAUGAAGAGGAAUAUGAGUUCUGCAGCAUGAUUGUGAG
GUUUUAAAAGGCUGAUUUGUUCAAAUCUUCAUGAUCACCAGAUGAAAGGUUAAGAUCAUG
AGGAUUAUAGUGAUGCAUUUUUAAACUAAGAUACACAUUCUUGACAUGAUUCUGAAAAUU
UGUAUGAAAGAAACAUAUCAAUAGGUACUACGUUGAACUAAUUCCUUUACGCUAACAAAA
UCAGUUUAAAGGAGAUUGAGAUGCUUUAUCAAGAAGUUUAUUUUUUUAAGUUUCAAAUGA
U

2. MFE structure-
...((((((((.(((.......)))))).(((((((........((..((((..(((((.((((........))))..................(((...(((((((((((((.((((....)))).)))))..))))..))))...)))(((((......(((((((...........))))))).......)))))..
(((((((((((((((((.((..(((((.(((((((.......(((((((.................((((((..((.....(((((.....)))))....)).....)))))).......(((((.(((((((((((......(((.((((.((.....(((.((((.((.((((((........((((((.........
..(((((((((((....)))))..))))))..........(((((...........)))))...((((((......)).))))....(((((...((((((((((.((((((((((((((.((((.((((((.(((((((((....)))))))))..)))))).)))))))).......((((((((....))))).)))
(((((((((((..((((......)))))))))).)))))........((((((((......))))).)))......))))))))))....))))).)))))..)))))......))))))......)))))).)).))))...............(((((((((((.((((....(((.((((((((..((((((((..(
((((((........((((((((((...............))))))).)))........))))))).))))..........(((((((((((...........)))).)).)))))...(((((......))))).))))..)))))))))))............)))))))))))))))....(((((((((((((((..
(((((............(((((..............))))).....((((((((((............((((.......))))...........))))))))))...........))))).))))).)))))))))).........))).....)).))))..)))....))))))))))).)))))........(((..
(((((......)))))...)))..(((((.((((((((((((.(((((((((.(((((.......((.(((((...((((..(((((.((.....((((..((((((.((((.....((((.((..(((.(((.(((..(((((((((......(((((........)))))......)))....(((.(((....((((
.(((....)))))))..))).)))........(((.((((.(((((((((((((((((((....(((.((((((((((((((....(((((....)))))......(((((((.((((((...(((......)))....)))))).................(((.......))).....))))))).))))))))))).
....))).)))..))))))))))).....)))))))).)))).)))......)))))).))).))).)))..)).)))))))).))))))....))))....)).)))))))))...))))))).......)))))))))).((((((((((....((((...((((((((((((((((........)))...)))))))
))))))))))....)).))))))))..((((.......)))))))))))...)))))).))).))))))))))))))))))))))))..)).))....................))))))))))))))).)))))..)))).))......)))))))............))))).......

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-450.41] kcal/mol.

2. The frequency of mfe structure in ensemble 2.06748e-29.

3. The ensemble diversity 542.89.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS