lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-407.30) is given below.

1. Sequence-
GUAGAUCAUUUAAGUUGUAGUGCUAACAUAGGACGGGCUUUUGCAUCUAAAAGAAAUUAC
ACCAAGGCUACCAGGAAAACGAUAAGAACCAUAUCAUAGAAGUUUAAUAUAUAGAAAGAG
AUAACGAUGAUUUUUAAAAAAGAAAAAGAAAAAGAUGUAAUAUGAUUUUGUAAUUAAACG
GCCUAUUUGUGGUCUGAAGUUUGGUUGAGCCAGUCUUGUAAGCAACUUGAGGUUUCGGCA
AUGAGUUCACCUCCUGGUCUGGCUAGAGAUCCGCUUUUUGAUAUCAGUCUAGCAUCUUUC
UUCGUUUGUGCAAUCUGCUUAAGUGACAGCUCUUUGGUUACCGUUGGCUUCAGAGGAAUG
CUCAAAAUGUGUGUUUGAUUCGAGCCUCAUAGGAUGAGGCAUCUCUGCUAAGUCAUUCGC
AGAUGAAUUUCUCUAUUAUUAAUACCAAUAAUCCCUUCCAUUGGGAUAUAAAAAGUGGCC
UUUAGAAUGACUCAUCCUAACAAUCAUUCUGCAUCCAAAGACAAAAUGAGAUGAUGAAUC
AGCUUGAUUCCUCCUCAUUUUCACCGCUAAAGUGAGGACGUGAAGAUGAAUUGUUCUUAU
GUCUCCUCAUGCCAUCUCACCUGGGGAACCAAACACGCACAGUAUAUACAAAGAAUCUAG
UCAAACCAUAUUAAAAGAAAAGCCACUCAAGGUUAACUAAACCCAAAGAGCAGAGGGCAA
UAGCAGGAAUAUAAUCACAAACAGUGAAUAGUUGAACCUAAGAUCCCUGAUUUCAUAUUG
ACAACGAGAGGAUGAAUUUAAGGACUUCAGAAGCCUAUUUAGCUCGACACAAUGUGAAUC
AUAUAUCGAAAAAGACAGCAUAAGAGGUCAGGAUCGUGGACCUUUCUAAAAAACAGGUAA
GGGAUUAUGGAAGUGCAACAGAACAAAGAAAAUAUAUAUGGAAAUAUUAGCUAAUUAACU
AAAGUUUUGGUCUUCGGUUAGCAGUUGGCAGUUCGUUCCCCUUCAAAACGACUGAAAAAC
CCUAAGACAGGGGGCAUAUGAAGAACCAAUGCUAGGUGUUUUUCUGUAACAGUUUUUCUU
UUUACUUACACUUAAUAAAGAAUGCCCAAAAGUGUGAGAGCAUACACAGAGCAUCAAGAA
GGCAAAGGUAAUCUAAGAGAUUAGAGAUCUUGCAGUUAGUUAAGCGAGGGACAGCUUUCU
CCGGCUAUAUCAAGUUUAGAGUGAUGCAAUUAGCCAAAGAAGAUAGAAAUGUGUUUAUUG
CUUUUACCCCAUAAUGUUCCAACAAGUAAUACUACGCUUUUAAAAGAUAUAUCAGCGUAA
AGCCGUAAACCUUCCACUCACCUUCAAUUAGAACAAGGAUAAACAAAAUGUAAGUUCCAU
GUAACCUAGCUGCCUACUGCAGGAUUUACUGGCCUGACAGGAUACCGGCAAGCUGCUACU
GCAUGUGAAAAAAGUGGAUCCUGAUGUUAUUGAACCUUUUCAAUCUCAAAUACUGUUACU
CUUGCAGAUUUACGGACAAAGUUACCUGUCCCUUUAACACUAUUUUGCUGCUAUCUCCUU
CUCCUGAUAUGGAGAUCAUUUGUAACUUAAACUUGUUCACUGCCUUGCUCCAAGGUGUAC
AAGCACAUUAAGUUCCGGGCGUUUGACAAAAGAAGUUUUAGAAGCUGGUUUACUUGUCCC
AAAUCUUUGAAAUUGCUCAAUCUCAACUUCAAUUCAACAGGCAAACUUCUCUUCAAGUAU
UAUUCCAUUGUGCAACAGCAUUAAAG

2. MFE structure-
................((((((.(((....(((((((((((((....))))))............(((((((((((((((....(((.(((((..((((..(((((((.(((((((....((.((.((..(((((............)))))..)))).))....))))))))))))))...)))).))))))))...))
))(((.((((((...(((.((((........)))))))...)).))))))))))))))..))))((((((..(((..(((....)))...)))))))))(((.(((((((.(((((((...(((((((((((((.(((((((.(((.((((....((.(((((..((((((.((((((((.(((((((((...)))))))
.(((((((.((....)).))))).))........................(((((.......)))))........(((....((((((((.............))))))))...))).........(((((((..((((((...)))))).((((((((((.......))))))))))(((((.((.((..((....)).
.)))).))))).)))))))..(((....)))........................)).))))))))))))))(((((((((((..(((..((((.....))))....)))..(((((........(((((....((((.....))))...((((.(((((.(((((((((((.(((((((....((((....(((((...
(((.(((...))))))))))).))))...))))))))))).....((......)).......(((((((.........)))))))..............))))))).)))..)).)))).((((......(((((........))))).(((((((((((.(((.......))).)))))...)))))).))))))))))
))))......((.((((((((((.((.((..((............))..))))))).))))))).))....)))))))))))..(((((((((................)))))))))..........)))))))..)))).))).))))))).))))).((((((((((((((((..((..((((((((((.(((((((
..((((((((((.........)))))....)))))..)))..........(((((..(((.....((........)).....)))..)))))((((((..((.......))..)))))))))).))...)))))..............(((.(((.(((...(((...)))....))).)))...))))))))..)))))
))))))).)))).....((((((.(((....(((((....))).))........))))))))).((..((((((....))))))..))....))))))))...)))))))))))))).)))...)))))))..))).))))))..(((((.........(((((......)))))........(((((....(((((...
(((((.(((.....((((....))))....)))...)))))...)))))...)))))...((((..(((((.(((((.........(((((.(((........))).)))))....))))).)))))...))))..((((.........))))..)))))......

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-441.26] kcal/mol.

2. The frequency of mfe structure in ensemble 1.16749e-24.

3. The ensemble diversity 361.46.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS