lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-326.24) is given below.

1. Sequence-
CGAACACCAAAAAUGAUGACGAACUAUGUAACUUUACAGAUUCAGGUGCUCAUGUAUAUG
UUUGUCUAGAUUUUGUUUACUCAUUCAAAGUCUAGACAUAUUUGAGAACAUCAAACCAUU
UUAUAAUGCAUAAUAAUCAGUUAACACUAAAAUUGUAACAAAAUUGAAACCUCACCAACA
CAGUUCCAAGCUAUAGCAUUCCGUUCAAGCAGUUCUAUGCCUCCGUCGCUAGUUACUGAC
AGAGACUGUUCACAGUGUAGAAUCACAUUGCAAGAAUGAGCAGGAGAGAGGCAAAAUAGU
ACGGUCUACCCUACAAGACAGAAAAUUGCUCUCGUGUUUUGUAUAUGGACAAUGUACCCA
UUACAUGGAAAGCAGUUCUUGUAAGUAGGAUUUGAUCACGGGUAAUAGUUAUAAUUGUAA
GAGAAAGUUGCAACUUAGGGAAUGCAGGUGGCAAUAUGAAAUGAAAAAGAGUAUGAAUAA
AUUGGUCUAGCUGGAAACCAAUGGUGGUUUACAAGUAACUGAUACCAAAGGAUCAUUGCA
AAAAAGUAAGAUACAGAGCAGGUCAACAGCUUGUCCGAUGAAAGAGCACAUGUGGGCAAG
CCUUCGACCAUUUACAAAGAACAGCAAUAAAAGAGUUCCUUCACCUCAUGACAUUGCCCU
GGUCCAAUUGGUAGACCAUUGUAUGCUAGUGUUUAAUUAUUCUUCUAGGAACAAGUGUAG
AAGUCACACAUUUCUUAGCACUCCCAGAUAUCAUAGAAGAAAUGAAACCGAAUUGCAUUA
CUUUCCCCUAACAAACUUUAUCUCAGUUAAUUGUUGAAAACCCAAUCUAAAAAGAUUCUC
AUAUUUCCUCUCUUCUUUGCUGUAUAUGUAAUACUUAAGCAUAGUUCUGCAGGAGUCUCG
CAAACUAUCUUUUCUGUUCGACAAAAAUUCAAAUUUAUCAAUUUAGUUGAACUAUUUUUA
UAACAUUCUCAAUCGUAAUACUUUAUUAGCUUCUUAAUUUUUUCCAGCAUCCUCCCCUAA
UUAAUACUAUAAUAUCCAUAACACUCGAGGUACCUCUUAUCCCAAUUUAUUCUCAGGAAG
AGAGUCUUUUUUUAUCUUCGAUUCUUUUAUACCUGGGCUUUGCAUUGACCCCUUCACUUU
UAAGUUCCUUCUUUUCCGCGAUAAGGUAAGGUAAACUUCUAACUUCAUCGUCUCUUGGAU
GUUUAAACCGAUGCCAAAAAGUUUCUAGAAUUUAAGAGCAAUACCUUUUCUUUUAUAAUC
GUGAUGUCUGGGCCAGCUUUUGUACAUCUCGACUAAUUCGAUAGGAUACCUACCACCUCC
CACCAACAAUUAUCAGGUAACUCUUCCAUCAAAGGCUUAGAAUAGAUGAGAAGAAAUCAC
CUAGUGUUUUUGUCUCUGCUCAGAAUUGAAACCGAUACUUCAUGGUGCUCAACCCACGUC
AUUGAUCACUAAGUCAUACCCUUGGGUGCUUUAAGACCGAUACUAAACACACUAAAUUAU
GCUCUCCCUUUGUCUUUCUUUUUGUUUUUUCAGCUGUCUCCUUAGUGCUCUAACUCUAAA
AGUAAAAAAUUAAUUAAUUAAUUAAUAAUAAUUGAUUAAUAAAUAAUAAUAAUAAUAACA

2. MFE structure-
.(((((......((((..((......((((....)))).......))..)))).....)))))((((((((((((..........))))))))))))...(((((.....))))).((((....))))..........(((((.((((((((((((........(((....))).....))))))....((....))...
.........((((.(((.((.......)))))..))))...(((((.(((..((((((......))))))...(((.((((((((((((((((((.(((((((((((..(((((...((((..(((((((.((((((...((((((.....)))))).....))))))..))))))).))))..))))).....((((((
.............((((((((((((((((((.....(((((((((((..(((......................(((((((((((....(((((((((((....))))))....((((.((((.((...))))))))))((((((.(((((.....(((.((((....((((((((((((........))).))))))))
)....))))..(((((....(((((((...((((((((((.......((((....((.(((((.((((.((....))))))...))))).))....))))......)))))).(((((((((((.....))))))..))))).............((((.((((...........)))).))))....(((((((((...
....)))...)))))).......(((((....))))).............))))..)))))))...))))).....(((.((((((.(((((....)).))))))))))))....(((((((..(((((..........))))).)))))))......................((((....))))..)))))))).)))
))))))))..((((................................))))...........)))))))))))((((((((((((((.............)))))))))...)))))))))))))))...))))....((((((((((...(((((..(((....(((....(((((.(((((............))))).
))))).)))..)))..)))))......)))))))))))))))....))))))))))))))))))).(((((((.((((((((......((((.....))))..(((....((((.....................)))).....)))..))))))))..........((((.((((.(((...))).)))).)))).)))
))))..(((.((((........))))..)))(((((......((((......))))......)))))......)))))).))))).......................)))))))))).)))))))).)))))).))))).))))))...))))).............((((((((((((........))))))))))))
....................

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-357.53] kcal/mol.

2. The frequency of mfe structure in ensemble 8.88599e-23.

3. The ensemble diversity 354.50.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS