lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-481.30) is given below.

1. Sequence-
CCCCCUCUUUGCAUUCAUUGCUUCUUUCAUUUGAUUGACCAAAAUUCCAUAGCUAGCUCA
UCUCAUAAUGAGUUGACAUUAUUUGGCAUGAUGACCUAGCCUCUCUCCUGAUGUUGAAAC
UAAUGCUAUUGUUUUCCCUGUGCUUCCUUUCCUCACAACAAAACAGCUGAGAUAUAUGAA
GAAUGAAGCCAAAUGAGGAUUUGAGAACUAUGGCCUACCUUGAUGCUUUUCCUUAGGAGG
ACCAUAACUGAAGAUUUUACCUACUAUCGAAAUUUGCCAUGUCCUGACACCAUACAGUUA
CAAGCUCAAAAUCUUGUGGUCCCAAGAACCUAUGUAGCCGUGCGCCUUUAUCCUUCUUCC
CUUUGUGUGGUUGUAUUGCAGAUGAAUUAAAACUCAAAUCUCAGCCAUGUCUUGAUGUCU
UUCUUUUUCCUGUUUAAUUUUUACUUGAUGAGUUAUUGUGGGAGUUUCUUAUUUGAUGAA
GGUAGAUGUUAGACCUGUUGUCCCUUGCAGUUCUUUCUAGUUUUGCUGGUUCUUUAGAAU
GGAAAAGUUAAUUUGUACCCUGAUAUUGGAACGUAGGUCAAUGUAUCAUAUCUUUAUUUU
UGAAUUUCGUUUCAUCUUAUCUUGUUUGCAUCUGAUAACCCUGAGUGUUUGCUCAAGAUG
UGUUCAUAUUCAAUCAUGAUUUGCUUCUCUGACCAAUUCAUUGGCAAAGCACCUUCUGAA
CUUUUUGAUGAUUAUUUUGAAGAGGAUCUCGUGGAUACCCAAGAAACAUGAAACUUGUGC
UGAAGGUCUGCCACAGUUAUCGGUAGGCAGAUUUUAAAUGUAAAUGAAUAAUGCACAAUA
UUACAGUAACUGCAGUAAGUAUAAAGUAUCUGAUGUAACUUGCAUUGCCAAUCACCGUUC
ACCUGACAGUCUCUAGGGAGGAAUACAUUACAGUAAGCAGUAAAUCUGGCGGCAAGUUUU
UAGUAUGCUUAGAAUCAUGUGCUAUGUUAUGAUUUAGUUUGUGCGCAACCUAAGAUAUAA
UUUCAUUAACAAUCAGAUUUUAACAGAUCUCGCUCAGAUAUGCACACAUCAUAGGUAUCU
CACAUUCUCUGUAUUUUAUAUGUGAAGAAUAUCUUUUACGAUUACAAGAAGCUAAGUGGU
UUCUUUUCCACCUUUUUUUUCUUGGGGUUGAUUUAUUUCUGAUGUGAAAUUUUACAAUGC
UGUAACCGGGUAUCGUUGAUAGUUCAAAAUUGUUGCAUUGUCAUUCUAAAUUUCACCUUG
CACUUCUCUCAUUUCAUCCCUACUAUUCUCAAAUGCGGUGCUACCGUUUCUGGAAACAAU
GUUCCAUUUACAAAAUCAUGUGAAGAACAGAUGGUCAUAAGGUGAAAAUUAUUUUUUGAC
AAACACAAUCAAUGGUGUAAAGGAAUCAGAAACUGAGGCGAGAUUUUGCUUUCUACCAAG
UACUUGCACCAAGUUGAGACUAUGUAGGAGAUGGAACUACCGAUAAUCAGGUCUGGAGGU
GAUGGGAGUCUCUAUAUAUAUGCAGCUGGUGGUUGGCUAGCUUGAUGGAGAUCUGAGCUU
AAGCUCCCUCAGCUAGUGUGGUUCUUUUUGUCAAAGAUAUCUUCAAUUGCUUUAGAUUAC
AUCUCUAAUUUAUUAAUGCAAUUGUCUGGUGUUUCAUAGGAACUAAGAGGAGGUUUUUGU
CAUCCUUGGUGACCAAACAAUUGCGUGGCAAGUUAUCUGCUGGGAGAUGAAUCUGUCUUC
UCAAUAGAAGUGCAUGAUUUUAAGGGUUAUAAAUCUAAAAUAACACGAACUUGAAUUAUU
GACUAUGGCAUGCAUAAUGAGCUCCCAACGCCUGUGUAUCCGAAUUGCUACAGAACAGUG
CCUUUGUGGAUGUCAAAUUAAUCCAUUACUAAGAACGUCUUUUCUACAAAAAAAGAGUUG
CUGCUAGUCUACAAUAACCCAUUUAUUGAUGCAUUAGUUGCGACCGUACGUGUAUCUAUA
ACCUAGAUGCAUUAGUUACAACCAUUAAUUUUACUUAUUUGAAAAACAUUAAUUCUUAUG
CAUUAUUUGAGUUUUAUGGUGC

2. MFE structure-
...((((((((..((((((.((((..(((......))).............(((((.(((((((((((((......)))))..)))...))))).))))).((((..(((.(((((........((...............))............)))))...)))..)))).....))))))))))..)))).))))..
........((((((((.(((.((......))...)))))).)))))((((.(((((((..........)))))))..)).)).....(((((((.......(((((((((.(((((......)))))......(((....))).......................(((((((((...(((..((((((.(((((((...
.((((...((((.(((((((.(((((.((..((..(((((((((.............)))))))))....))..)).))))).)))))))))))..))))....))).)))).....))))))..)))((((((.((((((((....((((((((.((...((((.(((((((....((((.(((.........)))..)
)))....)))))))...))))...((((((((.(((((((((((((....))))).(((((((.((((((.......((((((....(((((((((....))))...(((((.....((.((((((((........))))))))..))(((((.............))))).....))))).((((((((((((......
..)).))))))))))...(((.((((((...(((((((.((((..(((((.(((.........((((((((((...(((((((..(((((....((..((.((((........))))))))......((((........))))...)))))))))))).)))).)))))).........)))...)))))....))))))
)))))................)))))).))).)))))......)))))).......)))))).)))))))..(((((((((((((..............))))).)).))))))..........(((((((....((((........))))......))))))))))))).......)).))))))))...(((((....
))))).((((((((((((..((((((...((((.((((.(((((((.....((((((((((((((((....((((((((((((((.((((((....(((((....(((((((....((.(((((..((((((......)))))))))))..))((((.(((((((...))))))).))))..((((........))))..
......))))))).(((((((....))))))).............)))))...)))))).)).))))).)))))))((((((((.....((((..((((((((((((((((((......((((((((((.((..((((((((((((....))).)))))..)))))).)))))).))))(((((((..((....((((((
(..(((((((..(((((((......))))))).....)))))))...))))))))).)))))))....)))))))))))))).))))...)))).....)))).))))..(((.....)))(((((((......)))))))....)))))))))........)))(((((.........))))).......)))).....
))..)))))))))))))))))))..))))).....))))))).....(((((((........)))))))..)))))))))))))))).)))))))).((((((.......))))))(((((.((((((.((((((((.....)))))).)).)))))).))))).....(((((((((.....)))))))))....))))
)))))...(((((......)))))..(((........))).....)))))))))))))))).

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-519.51] kcal/mol.

2. The frequency of mfe structure in ensemble 1.18014e-27.

3. The ensemble diversity 677.89.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS