lncRNA Structure Prediction

Species Name : Withania somnifera Sequence Id : GBHJ01020458.1

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-585.40) is given below.

1. Sequence-
AUACAAUCCAAAUCAAAAUAAAAAAAAGGGAAAAGAGAGUAUGAAUGUCGUUAUAAACCC
AAAUAUUAAAAUAACAGCAGCUGAGAGAUAAAUACCCCUCCUAAACUAAAGAUAUACAUU
UUCCCCAAUGCAAUAGACUAAGAAAAGUAGGGAAAGGAGUAGAAAGGUCCCCCUGUGGUC
AUCAAGGCUGAGGUGGAGGUGGGGCAUCAGUGCCCCUGUCCUCUGUAGGCGUUGUGGACU
UGUCAAAUGUCUCCUCCGCUGUCUUGAUCCCUCUAGUACCCAUCAUGGUCAUAUCAUGAA
UCAGGUGGGAAAGUCCCAUAGCUAGGAAUUGUUUAUCAAGAUCCUACAAAUCUCCAAAUG
CCUCAGUCUGAUUAUAUCCCACCAGCUUCCUCAGUCUGUACAGUGUGCUCCUGAGUCUGU
GCUACCCAGUGAAGCUCCUACGCAUAUGGGGGCUUUGAUGGUGCUGGGACCCCAAACACU
UGUGUCAAGUCCGCUGCUGUAACCUUAGUCAAUUCAGCCCACAACUAUGUUGUAGUGUCU
AUCAUCUCUUGUCAAUCACGAUAUGUAAUAACCAUACCCCAAGCUUGGGCCUACUGUCCC
UCUCUUAUUGUAGUCCUAAGAUCAUCGAGCCUUGAACCAUAUCACUAAACAUUCAACCUG
AUAUCCUGAACUUCCUGCUGAAGAGGUCCAACAAUGGUAUUCACCGUGGCGGUGACCAAA
AAGUCCAUGUCCCUCUCUAGUAUAAUAGCCGGCUGCUCUGCUGGUACAACAAGUUGGCCU
AUAUGCAUAAUCAUGGAAGGACUAAGGUGGGACUCAUAUUUAGAUAGGGUCCCUAUGGGG
CUUGGUGUGCCUUACUGUGGGAAUGGCCUGACUACUGGGUAAAUGGGGGGAGGCCUAACA
GGAGAAGAAGACCGAACCUGUAGGGAAGUUAUGGGCCCGCUAGGUGGAAAACUGGAGGAG
GCACAGGUAGAGGAAUCUCAGUACCAAAAGCAACAAUAGUCACUUGUGGAUUAGGUAGAG
GAACAUCGAUUAGUGGUAUUACCUUCUCUCUUGUAGGCCCUGACUCAUCGCCACCCUAAG
UAUCAGCUUUAAUCUCAAGCUUAACCUCAACAAAUCCUCCAAACACUACCUCAUCAAUUA
ACCUAGGCUGCUGUUGCUGCUCAUCACGUAUCGCCUCCUCUAUACGAGUGAUAUCUACUG
GCAACUAGCCUCGAACUCAAUAUCGACUCCCUUUGUCCUAGGGACUCAUGCCCUAUGGCC
CAACUCUUAAAUCAAGCACAGGAAAUCAUAUACAUAGUCUCCUUAGCACUCCGAACCCUC
UCAACAAGCUCAUUCUAUAUAAUCAUCCCCAUAUUGAUCAAGUCUUGAUCAAUAGUGGAU
CCAAUGAUGAUUGCUCUAUGGAAUCAAAUAAUAGACUCAUUCAGUGAGGGUGCCAAGUUG
GCAUUGACAAAUGUCAACCAUUAUUGAGCCAUUGCAUGAAUGUGGAAUUUCUGAAACUCA
UUUCCUUAUCAAUUUAGGGGGUCUCCCACCACUCAUUAACUCGAAUAACCAACCCCACUG
AUCAUCAAGACUAAUCUUCAUCCGUUCAUCCAUGUAGUACUCAUCCAUCAUAAUUUGCCU
CCUAAGCAUUAAUAAUAUCAUUAACACCACAUGGUACCUUGACAACUAUGACUCUAACAA
AUGUGGGGUAAAUUAAAUGGGCAUCCUUCUUCUUUGACUUUGGUGGGACAACCUUUUCAU
AGGCGCGAUAGAAUUCCCUAACCCAAGUUGGGACGAACUUGGGCAAGGUCUAGUAAAUCU
GGUGAACUCUUGACCCUCAAUAUGCCUCAAAAUAUUUUUAUAUUUUUCAUCCAAUCCCAC
AAGCGACAACCUUUUUUCCUCAAUGAUGGUUGUUGGCCCGGUACCAUGAAUCAAUUUUGU
UUCCUAAUGGCUUUGGUAAAGACCGAAAACUCUUUCUUUCUUUUCUUCUGUGUGGCCUCU
GGUGUGGGUAGAGGAGUGUGCACUAGAGUAAUAGCCCCCUGAACUGCUUCCCUCUUUUCU
AAUUCUAUAACCAACUGAUGCAAAAUGGGGAAGAAGUCUCAGGGUAAGAGAUGAAGGCUC
AGGCAGCCUAAUAGGGGCCUCAGAGUCAGACUACUCAGUAGACUCAUCAAUUGCGAUGCU
CUUUACCUUCCCC

2. MFE structure-
...........................(((((.(((((((((...(((.(((((................))))).))).((((((..............((.......)).......(((((((((.(((((((((...(((((((.(((((.((.(((..........(((.((((((((((.(((((((((((((((
((((((....))))))((.(((....)))))(((((((((((..(((((..((((..((((((((..((((......(((..(((((((...)))))))....))))))))))....))))).))))..)))))..)))).))).))))))))))....))))))))))))).........(((..((((.((((..(((
((.((.(((((.........((...(((..((((((((((......))))))))))..))).)).(((((((........((((...(((((((....)).(((((((((..((((.....(((((.((((((.(((.....((.....)).....)))((((((..................((((((.((((......
.........)))).)))))).(((........)))..))))))........((((...........)))).....(((((.(((((..((((..(((...((((((...)))))))))....))...))..))))).))))).....((((((......)))))))))))))))))((......)).......))))..)
)))))))).)))))))))........)))))))...))))).)).)))))...)))).)))).)))...)))))).))).....((((((.((((..(((((((((((..((((((((..(((((((((..((((((((((..((((.....((((((((((...(((((((....)))..))))...(((((((.((((
(.......(((.(((((((((.....((((.((((((..(((..........))).)))...))).))))....))).......(((((.......)))))........................)))))).)))..........))))).)))))))..............))))))))))...(((..((((.....(
((.....)))((((........)))).(((((.......))))).((((((...(((((.(((............((..((((................))))..)).......((((((...........((((((((((((((((.(((((((((((((...))))))))).)))).......))))))))...))))
))))..........(((.....))))))))).....(((((((((....))))))))).....))).))))).))))))....((((..............))))...........(((((((((((((((.(((.............((((((((((((((.....((((((.....................)))).)
).....)))).....(((.......)))(((((......))))).....((((((.........))))))............)))))))........)))..............)))...))))))))...(((......)))...((......))(((..((((((((......))))))))..))).((((.....))
)).........))))))).))))..)))(((((((....)))))))..........)))).))))((((((................)))))).))))))..))...............)))))))..)).))))))(((((..((....))..))))).)))))))))))..))))(((((((((.((......)).))
))))))).......))))))..))).))))))))))))))...)))))..........))))...)))))))))..)))))).((((..(((((.(((((((((..(((...))).))))..)))))...((((...))))..)))))..)))).)))))))))...))))).

You can download the minimum free energy (MFE) structure in

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-625.44] kcal/mol.

2. The frequency of mfe structure in ensemble 6.12587e-29.

3. The ensemble diversity 461.53.

4. You may look at the dot plot containing the base pair probabilities [below]



Download Dot plot

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot

* Results have been computed using RNAfold ViennaRNA-2.5.0.
* An equivalent command line call would have been RNAfold -p -d2 --noLP < test_sequenc.fa > test_sequenc.out
* A dot plot into the mountain plot coordinates transform using mountain.pl test_sequenc.fa | xmgrace -pipe