lncRNA Structure Prediction

Species Name : Withania somnifera Sequence Id : GBHJ01022153.1

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-320.12) is given below.

1. Sequence-
UCCGUACAAGACACUUGGACCCGGUUGUCCUUCUCUGUACACUUGGGAGAAAACCAAAUC
CGAUACAAUAUCAUUUGUACCUAUCCUAAUUCUAAUAAUAUUGGGCCUUGCACCCAUAAU
AUUGAAGUGAAAUGCGAUAAAGAAAAUAUUGGGCCUUAUGGCCCAUUACAACCCAAGAAU
AAAUGACAUAAAAAUGGCUUUUGGGGCUAGAAUACCGUUGCCUUUUCUUAAUGGGGUGGG
UUCUGGACCAUUUUUGUCUUGCUGUCCUUGUCUUCAAUCUAUAAAGGACUUGGCAUGGAC
UUUUGACUCGAUGAAUGCAAGUAAGAUGUCUGUAGGGAAGUCGGAAUAGAAGUGUUCAUG
CAGAGAAAAAUGAGAAGGAUUAGAAUAAAUCUUAUCAUCAUGAAGAUAAUGAAUCUAUCU
UAAACUCAAGAAUUAAAGUAAUCUCUCAGUAAGGUUUCAAGCAACAAGAUUUCCAAGGUG
ACUCAUUCAUUUAAUUAUUUUAAGCUGAUCACUUCUCUAUAAAAUAGGAUUUUUCCCAUU
UCAAGCCACAGCACUCAAAAUAGCAAAUAACAAAGGCAUGAAUAGGAAGACACUUCAUUG
AAACUACUGAAGAUAAAAAUUAGGUAGCUUUUGAUAUCAUUUAUCCUUGACAGCAUGUAC
CCAACUAAGCAAAUCAUCACUUUUCCCUAAAGCACACAUGUUCAAAUUACAUCCCUAAUA
AAUAAUAAGACAAUAUGUUGAAUCCUCUAAGUUAGUUUAAACAGUAAGCAGGUGAGAUUU
AUGCACAAAUUCUAAACCAACUUUUGUCAAAUAGGUUAGAUAGACAUAACAAUCAAAAUA
UCCAACCAAUGACAUCACAGAGUCCAGUUUUUAUUGCGCAGAUAUUGACAAGAUAGUCUA
GGAUAAAAAAAAUUAUCCUCUAUUAAACUUUAUUUUCAAGGUUAUACUUUCCCUGUUUAA
CAUGCAUAUUUAGGCCAUGGUGCAACCAACAGCAAACUAGAGCACAAAGAUCAAAAUUUA
AGGCUUGUUUUCAUGGUAAAACGAGUCACAAGUUGUACUGCGAGUUAGUCCAGUCUCCAG
GUUUUAAGCUAUAUUGAUAAGUCCUCCUUUUGAGGAUAACCAAUGAAAACAUAAAUUGCC
AACUAACUAAGUCACAAUCAUCAUGAUUAUAAACUAGUUGCUUAGGUUCAAAUGUUCUGA
UAUUCCCAUACAAUAUACUCAAACUACACUCCCCUCUAUUCAGAUAUAUCCAUCAAUGCA
UGGUAAAAAAUCUUUAGACAACACUCAAAACCAGUAAACCUGAGAAAAACUUGAGCCAAA
UUAUGACACCAAAUCAAGUAGGACUGAACAUGUUACAACUCAGAACAAGCCUUUUACAUG
CUUUCACUACAUACAAAUUAGCCCUAUUUUAUUAUGUAGUAACACGCACUUUAACACCUC
UUCUUUUAAAGGGUCUACUUUAUCACACAAAAGGGAUGAUUCAAUUAGAACAUUGAACAU
CCUAUCCACAUUAAUGUUAAACAUGAGAAAAACCUCAAGUUGUACUGAUUUUUAAAAUAA
AAUAGCCUAAUCUUCUAGGAGUCUAGGCUCACACUUUUG

2. MFE structure-
(((.((((.((((((((((...((((....((((((.........))))))))))...)))..(((((......))))).....((((((.........)))))).((((((..(((......(((((...((((((((((((......((((((....))))))......(((((((....................))
))))).(((((((.((...(((((........))))))))))))))....)))))))).....((((((..............))))))...))))..))))).......)))..)))))).))).)))))))).)))........((((((((((((((.........((((..((((((......)))))).))))))
)))((((.....)))).((((......)))).........((((.(((((..((((((((.........(((((.((((((.....((((((((.....))))).)))))))))............(((....)))...((((.(((...........................))).))))..)))))........)))
))))))))))))))(((((((((.((((((.(((((......((((((((.((((((((..................((((....((((((.....(((((.............(((((.........((((...))))...........))))).........((((((((.((((....(((.(((.....((((..(
(((((((((....((((.((((................)))).))))..)))))....)))))...))))...))).)))......(((......))).(((((((......)))))))......((((((......))))))....)))))))))))))))))....))))))....)))).........((.......
.))................((((((((((((...................(((((((..(((.((((((((.((((..(((((((..(((((((((((..((((((.....))))))...........((((.....(((((.((((((.((...(((((...)))))....)))))))).)).)))....))))(((((
.......................................))))).......))))))..)))))..)))))))..))))....((((.............))))....((((((...................)))))).)))))).)).)))))))))).)))))))))))).)))))))).))((((((((.((((..
.....))))...))))))))............................))))))......))))).......((((((.((((((......))))))))))))..........((((...))))(((......)))))))))..)))))))))..........((((((..(((....)))..)))))).)))))))))

You can download the minimum free energy (MFE) structure in

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-347.96] kcal/mol.

2. The frequency of mfe structure in ensemble 2.41959e-20.

3. The ensemble diversity 363.58.

4. You may look at the dot plot containing the base pair probabilities [below]



Download Dot plot

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot

* Results have been computed using RNAfold ViennaRNA-2.5.0.
* An equivalent command line call would have been RNAfold -p -d2 --noLP < test_sequenc.fa > test_sequenc.out
* A dot plot into the mountain plot coordinates transform using mountain.pl test_sequenc.fa | xmgrace -pipe