lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-431.80) is given below.

1. Sequence-
GUAAGACUUUUCCUUAAUGUGCUAUUGACUUAGUUCAUUUGUCUUUCUUGUUCCAAGAUG
GAAGUUUAUAGUAGCUUUGCUUGUGGGUGUCCAUUAAAUUCUUCCAAGAUUGCAUCUUUA
UUAUCCCCUCACUCCGUUCAUAAGUGGUACCUCUUGGUUGCUGAAACUGAGUUAGGUUUA
UUUUGAAUGAUUGAUUGACAUGUGGGUGCCCAUCAAAUUCUAUCAAUAUUUUCAUCUUUA
CCACUCCCUUCAUCCCAAUUUAUUUGAUAUAUUUUGACUUGGCAUGAAGUUUUGAAAAUA
AUAAAGAGAAGACUUUUGAAACUUGUGGUCUUAUUCUGUUUGCUUUGCUUUAUUUUGAUU
GGUUUCCAUGUGGGUGUCCAUCAAAUUCUUUCAAGAUUGCAGCUUUAUUAUCUCCUCAUU
CUGUGUCACUUAAUUCCGUAUCUUUAUAUUUCUUGUUCCAAGAUGAAAAUUAUGUAGUUU
CUUUGCUUUAUUUAGCCUAUUUUUUCAUGUGGGUGUCCAUCAAAUUCUUCCAAGAUUGAA
CUUUUACUGUCCCCUCAUUUUUUUCAUAAGUGGUACCUCUUGGUUGCUGAAACUGACCUU
ACGUUUAUAUUUUGAAUGAUUGCCUUGUGGGUGUCCAUCAAAUUCUUCCAAGAUUCCAGC
UUUAUUAUCCCCUCAGUCUGUGCCUUGAAGAUAUUCACAUAAUUCCCGCAUCUUUAUCUU
UCUUGUUCCAGGAUGAAAAUUUUGUAGCUGCUUUGCUUUAGUUUGCUUAUUGUUCCAUGU
GUGUGUCCAUCAGAUUCUUCCAAGGUUCCAGCUUUGUUAUCCCCUCAUUUUGUUCCUUGA
UGCUAUGCACGUGUAUCCAUUAUCUCUAUCGUUCCUGUUCCAAGAUGAAAAUUGUGUAGU
UGUUUUGUUUUAUUUUGCUUAUUUUCCAUGUGGGCGUUCAUCAAAUUCUUCCAAGAUUUC
UUCUUUAUUGUCCUCUCACUCUGUUCAUAAGUGGAACCUCUUGGUUGCUGAAAUGAGGAU
UGUUCUGCUUGUUUAGCUAUAUUUUGAUCGGUUUCCAUGUGAGCUUCUGUCAAAAUUCCA
AACAGGUGAGAAUAGUUGAGAAUCUCUUGCUGGAAAAAGAGUAAGAACAUAGAUAACUAA
CUACUUUCUUCGGAUGACUAGUGGAAUUCAUUCAAUAAGACAGUAUUUAGUUGAAGAUGG
AGUUUAAAAGGUUAAUUUGGCUUAUCUAUUAGAUUAGUAAUAGCUCAAGGAAAUAUUAAA
GUAAUGGUUUAAAAGUUGGUUGAUGGAAAACAUCAAUGAAUUUGAAUUCUUAGAAGUUGU
AAAAGUUGGAUAGAAAUGAAUGGUUUCUAAGAUUUUCUGAAAUCCUGAGAAGAUGUUUUA
UGAAUUGGCAUGCAGGUGUACAAAUAGGUACUUAAAUGGUUUUCUUAUUGGAAACUAAAG
UUUUCUUAUCCUUCGAUUGAGUAUUUGGUUAAGAAAGGGAGAUUUGAGGGGACACUGCUA
AUUGUGCUCAAAUUUUGAGGACUUUGUGAAGGCUAAAGUGCUUUGAAGAUACUAGUUAUU
AUGCAUUCGUAGGUGCUUUUUUUGGUGAUCCUGAUUGCCACUUAUGAUUGAAAAGUAUAU
GACUAAUCUAGAAUCCGAAUUUAGUGUAUAUGAAUCCGUGUAUUCUGCUAAAGUAUAUGU
AUGAACUGUUGACAACGAGGACCAUGAAAUGCAAAGUAGUUAAGAGAACUUUUAAUUUGU
ACCGUCAAAAGGCCGUUGCCAAGUCUAGUCACAAACUUGAGGGAAGGCUUAGAUUAUAGA
GUAUGGAUGACGACACUAAGCUAGGAAAAACGCCCAACGUGUUUUGCACCUCGUUCAGGG
CUUAAGUGUGCUUUAA

2. MFE structure-
.((((.((((((((((((((((((..(((((.((......((((((...((..((((.(((((((((.(((..(((((((((((((...................(((.(((((............(((.((((.(((....(((...(((....))).)))..))).)))).)))...((((((((.(((((((.((((
((((((.(((.((((((......(((((((((((.............((((((.((((.(((............))).)))).))))))...))))))))....((((((((((............)))))).)))).......)))......)))))).))).)))))....))))).)))))..)).)))))))))))
))))).......(((((......(((((..((.(((((((((((((....(((((...)))))...((((...((((.....))))...)))).((((((........)))))).....((..(((((....((((((..........(((......(((((.((((((((((((..((..(((((((((((........
.((((((.....))))))...(((((((((....))))((((.(((((.((((((..(((...(((((((((((((((((((...((((....))))))))..((((...((((.(((...((((.((.((((((((((((((..(((......)))......((((((((((..(((.(((((((((((.(((((((((
..(((.(((((((((.(((..((...((((((((((((((.(((((.((.((..(((..(((.(((((((.((((((((....((.((((...((.((((((((((((((....((((((.(((((((.(((((.((((.((((........((((.....))))........((((((.(((((.(((((((((((.((
((.(((.....))).))))..)))))))))))........((((((((.(((((......)))))...))))))))....)))))))))))....))))))))..)))))))))))).))))))))))).)))))))))))...)))).))((((((..........)))))).....))))).........))))))))
))..)))..)))..)).))(((.((((...)))).))).)))))))))))))....))))))...))..)))(((((.(((((((.....))))))).)))))..................)))))))))(((((((.....)))))))(((...))).....)))..))))).)))).)))....)))))))).))).)
).)))))))).......(((((((.....))))))))))))))))..)))))..)).)))).....))).))))..)))))).)))))))))......))))...)))..))))))))))).))))..))))).(((((((((((((..................)))).))))))))))))))))))))..)).)))))
)))))))..)))))......))))))))))))))..))...(((.(((((((....))))))).))).))))).)))))...))).)).)))))..)))))))))))...)))))))..)))...))))))))).)))).)).....))))))....)).)))))))).))))...))))))))))))))).....((((
((..........(((((((((((((.(((((((.....)))))))...))).......))))).))))))))))).

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-467.34] kcal/mol.

2. The frequency of mfe structure in ensemble 8.99912e-26.

3. The ensemble diversity 505.33.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS