lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-400.50) is given below.

1. Sequence-
UCUCUUUGUAGCUUCUGCAGAACAACAUAACAAAAUUUUGUCUGAAAAACGUUCUCCAAC
GUUGCAAUUUUUUCCCGUUGUUGUACGAAUUUGUGAGCUGGAGAUUCCUGGAAGAUUACU
GAAGCUUCUCAAACGGUAAUUUUCCUUUCCUAAAUAAUUUUUUAAUUGAAAUCCCAUCGG
UUUCCCCUUUAAUUUGUUUCCCUAAUUUUGUUCUAUAAAUAACACUCACCUCUUCAUUAU
GAGGGUAGAAGAAAAGAGUAGAAGAAGAGCAGCCAAUAAUAUAGAAGAGAGCAGUUAAUA
UCAGCUAGUAAUGCAGGGAGAGUUUUAGGGUUCUUAGUGGGAAAUGACAGCAAGAAAUGG
AGCUUUAGGUUUAGACAUUGAGUUGUUUUUAGAUGAUUAAUAUUUGAGUUUCAAGCGUUU
UUGUAGCUGGACGAGGUAAAAUUCGAGUGCCCUCUAGUUCCUAUGACAUUUCUCUAAAGG
GGCUUUCCUUAUGUCUAUAGGGAACUAGAGAUUUUAGAGGUUUUCCUUGUUAGAUAUGUU
AGUUCAAAACUGUGGUGGAUGUCCUCAAUUUGUUAGUCUAUUUGGCUACUCCCAAAAAUU
UAAAAACAUCCUUCCAAACACUCUUAUUUCUAUAAAAUCUGUUUGGUUUGCUAUGUUUCU
GAUUUUAAUUAUGUGUCGGUUACCUGCUUUCUUCUUUUUUCUUUCAAUAAUUGAAAUGAC
AUCUUGAAAUUUCUAGCAAGUAAUAUGAAAGGUUAGUUGGAACUAUAUAGUUGUAAUCUG
CAUCAAAUGGCAUCUGAUAGGUUGGUUUUUGUUAUUGUGGAUUUGAAACUGAGUGUUGAA
AGUAUAAAUACAUAGUUUAAGCCAAGUAAUUAUGUUAAGAUUUUACCGGAAUAUGCCUGA
AAUGCUAACCUUACUUGACCAGUAAUGGAGAUGAAGGGUUAUGGGAAGGAUUAGUGGGUU
UGUUCCUCCUAAAAACUAGGUUACCUCUAGCAUGAUAGUUUGCCGUUUACUAGGAGUUAG
ACUCUUAUGCAAAAUAUAAAUUGUGUUUGCUGUUUUGAAUGUCCAGAAACUGUUUUGAAC
AGCACAAUUUAUAUUUUGCAUAAAUAUAAUCUGUGAGCACCAUACUAUCAUUUUCUUUGG
AUUGCUUAUUAGUUAGACAUUCCAAUGUUCUUUCCUAUGAUUUUUUUUUAAUUUAGGUCU
AUUAUUUUUUGUCUUUGAAAGCUUAGAUAAUGUGGCAUGUAUGUUUAGGUUGAAUUGAGC
AUUUAUGAGGUGUUCUGCUAUAGCUCCUUCUGUUUGAUUUUUAGUUGCAUAAUGUUUGCC
UACUGGGUGUACAACAUCUGUUACUUUUAUUGUAAGCUGAAACAUCCUUUGAACCUGGUC
UAUGAACCUUUCAAGUGCACUAUUUCUCUAGGUAAAUUGAUUGUUUAUAGUUGGACAGUG
UGUGUUGAGUUCAAUGUAUGUAUUUCUAGGUUGUAUAUGUCUCAAUUGAGAAUAGUUGUU
UGAGUUACACGGUUAUCUUGUUGAAAUGUAAGUAUAUGUUAUAAUUCAAAGGUUAUCUUU
GUGAAGUUGUGGAAGUCAUGAAUGUGGAAACGUGAAUCUAUAUUUUGACAUAUGAACUCC
AUAUGAUGACUUUAAGACAGUUUAGCCAUAGGUGGUAGUGGC

2. MFE structure-
.........((((...((((((((..............((((((((.......(((((...((((..((.(((((((..(((.((((....))))))).(((((.....((((((((((((............)))))))))))))))))((((......))))...((((((.....))))))((((..(((((...((
(((..((((.((((((........((..((((........))))..)).........)))))).))))(((..........(((..((............))..)))....)))))))))))))..)))).......))))))).))..))))....))))).......))))))))..(((.(((((((((((((((((
((.(((((.((((.(((.(((((.((((((((((((.((((((........((((((((((((((((((.......(((((....)))))))))).))).))))))))))........)))))))))))))........)))))))))).)))..))))....)))))..))))))))..(((((......))))).)))
))))))))..)))...((((((((((..(((((((((((((((.((..((((((......((((.(((((((((...((((.((.(((....((((((..............(((((..((...))..))))).............))))))..))).))))))))))))))).))))........)))))))).)))))
)))...........)))))))..)))....)))))))...........(((((.(((((((((((((((....((((..((((((..((......))))))))..)))).)))))))...(((((((((...((((((((((((((((((((((.(((......))).))))...(((((.....))))).((((((((.
((..((((((.((((((...))))((((((((((((((((((((....(((((((.((((((......)).)))).))))))))))))))))))))))))))).......))))))))..)).)))))))).....(((((.((((........)).)).)))))...))))))))))))))))))...........)))
))))))..............)))))))).))))).((((.((((((((......)))))))).))))...))))))))...))))....((((((.....(((((.(((.......)))..)))))(((((((((......(((...(((((..((((..(((((...(((..((....((((((((...((((.(((.(
((......))))))..))))..))))))))..)).)))...))))).)))))))))...))).......))))))))).((((....)))).........(((((((.(((..((.((((........))))))..)))..)))))))(((((((((..((((.((((......((((.(((((((((........))))
))))))))).....)))).)))).))))))))).))))))....(((((........)))))

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-429.86] kcal/mol.

2. The frequency of mfe structure in ensemble 2.03972e-21.

3. The ensemble diversity 468.86.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS