lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-285.48) is given below.

1. Sequence-
CACAAAGUAAAAUACCUUAAGGGAGGCCGCCUAUUCUGAAACUGAGGCGAUCAACAGAGG
AUAACAAAAGAAAUAAAGAAGAGAUCAUGCUGCUUUCAACCAUAAGACAAGUAGUAUAAC
AUAGUCCUAUUUGGUCUAAUUAUUCAUGAAAUACUUCCAACAAUCCAGUUUUAGAAGAAA
AGGGUGUAGGCAAUAUGUAGUAGGUAAAGUGAACCAAAAUAGUCAUCACCUAACCUCAUU
UCUAUUCCAGAUGCAGAGUUAUGUAAAGAACAAAAAUCAACAACCAACUGUGCAGCAAGA
AUAGUCACCUGACUUCUAUUUUACCUAAUACAUGCGUGUUCAUGACUUUAGAGAUGGUAA
ACAAAACAGGAAACAUGCUAACCGUUCACCUCGAAGUGUCAACUGGGAAAAUGCCCAUUU
UUCCGGUUCACACUGAGGAGCAAUCUGGCAACAAGGUUAAAGCAAAGCAUAUGAAAAGCC
AUUAACCAACCGUUUUACCCAACUGAGAUAUGUAAGAGGAAAGGGGGAACUUCAAGAUAU
UAUGCUCAUAUAUCCUGUUAAGACACCAAUAAGCUUCCAGCAGACCAAACAACAAAUAGA
CCAUAUGAACAAUCACUUCAACUAGAUUCAAUGUGCUUAUAGGUUAUCACUCAACCUAAA
CCGAUCAGAUGUCACACUAAAGCUCCUGGAAACUCAAGUUUCAGACUAGUUUUAACUUUA
ACAAACAUAUCUCAACCUUCGAUACACCACAUAGACCAUAACAUCAAUCAAACAAUAGAG
GCAAAUGAGACUGAAUAGAAGAAAAAAGUAUACUAGACAGAUUUUCCUAGAGAAAACUCA
AAGUACACAAAGGAUCAUGCUAGGCACUUCAAUGGUAAACCCUUAAAAAGGGGAGAACUA
AACUACUUAGGGUCAAACGACAUCGAAAAAUGGUGAUUUUAGAAGUAGAAUCAUAUAACA
UGGGAGGAAUCAGAUCAUAUUUCAAACCACAGGAGAAACUUACACACUCGACAUAACUGG
ACUCAUAAACAACCGUAUAACAUAGCUUCUUCAGCAAAAAUAGAAAACAAAGCACACUUA
AAACAUUUCCCAGGAAGGAUGAACGUCGACAUAAAAAAUGGCUUCUUGAACCAUCUAAGA
GAUGCAUUUUCACAACAAACAAACGGAUGAUUUACACAUCAUUUUCACACCAAUUAACAC
AGAAGUACGACACAAACUUUGAACUUCAACAAGCAAAUAGUCCAAUGUAGAUUCAACCAU
GAACUAAAAUAGAGCUUAAGAGAGGGCUUUGACAUUAUGUCUGGUAGUGAAAUAGAGAGC
UGUGUCAACAAAAUUCGACUAACAAAGAAAGUCAUGCCUAAAGUUCCAACAAACAACUUG
AAAAGAGUGAGAUUUUAUGAUUAACGGGGAAGCGAACCGGGACUGGGGGCUUGAAUUUUU
UCAAUUCUUUUGAGCCUUUUGAAAAUAGGAUUCAAACGAAGCAGAUAGACCUCUUCACUG
CUUCAAAAUGAU

2. MFE structure-
..............(((...((((((((((((............)))))(((.......)))..((((((((.....((((((((.(((((((((((........)).))))))))).((((((((((((((((((((.....(((((((.(((..((.(((.(((..((((...))))..)))))).))...((((((.
((((((((((((........(((((................((((((....((((.((((.(((...((.......)).)))...)))).))))....)))))).....))))))).)))))))))).)))))).)))))))))).........((((......(((((((....((((((((.....((((..(((((.
((((((((((((....)))))))))))).))))))))).((......)).....))))..)))).((((((((((...((.....((..((.((((((.............))))))))....))))...))))......))))))......))))))).....))))....(((...)))...............))))
))).........(((((...(((((((..(((((.....((((((.......)))))).((((....((((((....(((((((.((((((.......))))))...)))))))...............(((((..((((.....................................))))....)))))((((.(((..
.....((((...((((.((.....((((.(((....)))...........))))...))))))..))))...((((...(((((....)))))...))))..))).))))........))))))......))))(((((((....)))))))......(((((...(((((.((.......)).........(((.....
......))).((((...((((((.........((((........(((.....)))...................................(((((((........................)))))))...(((((...)))))..................))))(((((......)))))..................
.(((((.(((........))).)))))...........)))))))))).)))))..)))))........((((((((......)))))))))))))..))))))))))))))))).)).))))))...........((((.........))))..((((..((((((........((((.....))))..((((....))
))....((........))))))))..))))....))))))))..)))))))).)))))))......)))........(((((((..((....))...)))))))........

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-313.74] kcal/mol.

2. The frequency of mfe structure in ensemble 1.21721e-20.

3. The ensemble diversity 289.80.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS