lncRNA Structure Prediction

Species Name : Withania somnifera Sequence Id : GBHJ01027760.1

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-371.20) is given below.

1. Sequence-
AGGGUAUAGUUAGAAAACAUACUUAGUUUUGUCUUAAAACUCUUAAAGUGACAAUUAUUU
UGAAACUAGGGAAGUACAAUAUCAUCUAAUACAUUACGGGAUCAUAUGUUACAAAUAUCC
AAAUAAAGUAUUAAAAUUGAUGAUUUUCUAAAUUUGAUAUAAAAACUACCCUUGCAUAGC
UUCCAUGGCUCUCUAUCUUAUCUUUAAAACCAACACCGGAGGAGUUGAACACAGUGAAAA
GAGUCCUUUCUUUGAUGAAAGAUGUCACAAAACUAGUAUAAUUAUCACACACUUGAUCUU
UUUUACCCGUAAUAACAAAGCUAUAUUAAAAAAGAGUAUUUUUUUUUCGAAAAUACCUAA
AGGACAACUGUUUUUAGAAGAAGAUCAAAAUGGGUAAAACACUCAGAUUCUAGCAACAAA
AGUUGUAAAUUAUUAGUCGUUUUGCUUGUUACACCUUGACCAAAAUCUUAAUUUUAAUUU
UAACCGCCCCAGGCUACAUGACAAAACAUCACCUUAUGAUCCGUGGUUUGGCGUUCCCAA
UUACCAAGCUGAUUAACUGUAUAUACUUCAUAAUAUGUAUACAUACAUUUGAGGAAGAUA
CCUAAGAAAUAUGUAACUGGAAGUUUAGAACAUCAACUUUGAUUUUCUGGUACAUUCUUC
GGCUUGUUAAGAACCUAUGCAGCAGCAGAAACAAGUAGUCACCCUUUAUGCUACAAGAAA
ACAAAUUUAAAAUGGUAAGAAUAUACAGAAACUUAUUCAGUAGUAAGUCUAUCAUCCUUC
AUUAGCAUGAAGAAAUGACAAGCGAAUCUUGUUAACCAACUCUUGUUUAAUGCUUAAUUC
UUAUUUCCCACAUUGCAUGUCAGAUUAAGAAAAUGCAAAUAUCUAAAGGAGAUAAGACCA
ACACAACACACUUUCUUAGUUGACCACCAAUUCCCUUCCUAUGGCAAAAGCAGAUAAAGA
GCCCCAUCGAACAUUAAAAAGCAAUAAGUAGUAAAGUGUUUGAACUGCAGUAUACAAAGA
CUUCCAGGAUUUUGUAAAACUCUCUGUGUUGCAUUCUCUGCAAAUUUGAACCAGAGAAAA
AUAAAUACAUUUGAAAUUAAAAAAUAGAAAUCUAUGGUAUACAAUACAAAAAUAAAAAAG
AACAAAAAUGAUCUUCACAAAUAGCCUAAACUACUAUCCAAAACUAUAACCUAUAUAGCA
CCUCAGUUAAUCACACAAUUACAGAGCGGCCACACCUUAAAUUGCUCCUUUAUGAAUUGA
CAGAAAGGCAGAAGCAUAUCAGAUAUCGGCCCAUCUGAAUGAAUCAGAUACUUGGGAUAC
UCAACUACUCUGGAAUAACACAUGUAUCACAAUCUUUUUUUAUCUUAGAAAAUGGAUACA
CCAGCACUGGCAACCUCAAUUAAAUGUUGUAGUCUGAUAGGAUUUCACAUUAAAGCCAAA
AAUCAAGGGAGUCACAAACCGAAGGAUACUUUUUUAUAUGGGUAAUAGAUCAAAGUGUAA
ACUUUCUCCUUCUUGUGAAAUCAUUCAUUUAACGUUCCCUCAAGUUUGUACACAUAAUUA
AACGGUAACAAAGAAUGGUCGUACCUGGUCAGAUAGGAUUGAGAAAAGUUGCAUCAGAUU
CUUUAAUCUGACCUGCCAACUCAAUAAUACUUUACAAAAUCAUUCUAACCAUAAAUGAUG
AGGGAAAAAGAUAGAUUUUCAGCCAUAGUACCAACAACCAUCGGUGCGUGAUUCAAAUAG
AGAAAUGCACCAAAAACUAUUCAUGACAUGGAAAAAGAUAAUACCAAAUGCUCAUUAGCA
AUGACUGAGUGCAUUAAACAAAUAAGGAACAGAGAUACAAUUCAAUGAGGGGAAAAAUAG
AGCACCUUUAGAUUGGUGCAUUAAACAAAAAUGCAAAAAAUUUCAAAAAUAAAUUUUGUA
UCUGUAUCAAAGUUGUG

2. MFE structure-
((((((...(((((((((...((((((((((...(((...((.......))...)))...))))))))))...)).....((((((((((((.(((..((((..............))))...))).)))))).....)))))))))))))...............))))))..((((((((..((((((....((((.(
(((((.....((((.((...)).))))..........((((((....))))))((((((.(((.((((((....((..........((((..(((((((...(((((((((.(((.((((............((.((((((((.....))))))))))...((((....))))((((((.((((((((((((((((((((
(((..((((...(((((....))))).))))...))).)))))))))))).....))))......))))..))))))........(((...)))..(((((............)))))((((.(((((((((((......(((.((((((((..((.(((((..(((((.......((.((((((..(((.(((......
))).)))..))))))))..))))).(((((.((((....)))).))))))))))))..)))))))).)))......))))...((((.(((((((.(((....((((((((((.(((..............((((((.((...(((.(((.....))).))).....)))))))).)))...))))))))))...(((((
((.....)))))))....))))))))))..))))....((((((((((....((((((.((.......))..)))))).........))))))))))......((((...........)))).......((((((((....((((....((((.............(((((((((.....((.....)).....))))))
))).))))(((.(((((((.((....)).)))))))..)))...((((((...(((((((...........))))))).....))))))..(((((((.....((((....)))).((((...))))..........((((.((....)).))))......(((......)))(((((....((((((((.....((((.
.....))))........((..(((((.((((...........(((((((((.((......)).)))))....))))...........)))).((((((.....))))))..(((((...)))))...)))))..))......((((((.((...((((((......))))))))))))))(((....)))..........
.....))))))))..)))))))))))).......))))......))))))))..)))))))..)))).)))).)))))))))))).))))))).))))..........))..)))))).))).)))))).....((((((((.((((((..............(((.....((((((((.(((((((......))))(((
(((.......((((((((((....)))))))..)))...)))))).......)))...)))))))).)))))))))..))))))))))))))))))....))))))................(((((((..(((.....)))..)))))))........(((((...)))))..............(((((...((((..
....))))..)))))..................((((((.(((........)))...(((.(((((........))))).))).......((((((((...............)))))))).)))))).))))))))

You can download the minimum free energy (MFE) structure in

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-410.05] kcal/mol.

2. The frequency of mfe structure in ensemble 4.222e-28.

3. The ensemble diversity 466.58.

4. You may look at the dot plot containing the base pair probabilities [below]



Download Dot plot

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot

* Results have been computed using RNAfold ViennaRNA-2.5.0.
* An equivalent command line call would have been RNAfold -p -d2 --noLP < test_sequenc.fa > test_sequenc.out
* A dot plot into the mountain plot coordinates transform using mountain.pl test_sequenc.fa | xmgrace -pipe