lncRNA Structure Prediction
Species Name : Withania somnifera Sequence Id : GBHJ01033401.1
I. Result for minimum free energy prediction
The optimal secondary structure in dot-bracket notation with a minimum free energy of (-250.30) is given below.
1. Sequence-
AUAGCCGUUUUUGACCACCCCAGCCCCCCUUACCCCUAAUUUUUUUUCUCCAUAAAUACU
ACCAUAUUUCACUCAAUUUUCAUCAAAUUCUCUCAAUUCUCUCUACUAAUCAAACUAUUU
ACCAAUCUUCCAUUCCUAAUUCUUCAUUUAUCAAUUACAAAAUUCCCUACUAUUCAUUUA
UCAAUUACAAAAUUCUCCCAUUUACAAUUAUUAUAUUUUGUCAAUAUUUUAAUUUGAAGA
UUUGAAGCUAAAUUUGGUGCAAUUUGGAGGAGCAUUCUUCUUCAACUUUCGACAUCAAAU
UAAUUUCAUCUAUUUACGUCUGUUUUUACGCAGAAGUUUGGUACAUUCGUUCCACACUCU
CUAAUUUUAUUAUUUUAUUUUUGCAUUUAUUAAAUUUUCAUUCAUUUAUUGUAUUUGGUA
GUUUACUUUGAAUUUUAUUAAUAUUAUGGAUUUCUAUAAAAAAAGUUCGGGAAAGAAGGA
AAGUAGUACAAAAAAUUAGUUAGGAGGAUGUUAGGUUCAGUUCAUCAUCACAAUCUAGAA
ACCAAGAAAAUGUCGAUGUAGAACUAGCUAAUUUAGCCCCGCUAGAAACUAAUAUUGAAA
CUCCAGUAAUUGAUACCGAAGCAUUUGAAAGAAUGCACUAUUUUGAAGAUGUUGACGAAG
AAGUAGAAAUAGACGAGGAUUUAGACGAAACACCUACACCUACUAGUCCGCUACCUAAAU
GCCAACUCAGACUCCAGACUACCGAAGCGCAACUCAGGCUCCAAAUAAAUAUGAGCCCAG
GUUUGAGCUAGUUCCCGCCUGCUUCUCAAAGAAAAAGAAGGGGCAAUGUUCUCACUGGUC
GUCCUAAAAAAUCACCUGUAUGACAUUUUUGUAUUUUAAAUGACGAAAAAAUUCUUGCUA
CUUGUCGUUGUAAAAAAAAUUGGGUUUAUAAAUCCGGUGGCAGUGGGGGUUUAACGGGAG
GGAUGAUUAGACAUUUAGAAUCAAUGCAUAAUGGGAGCGACAAACACAUUUAACUAAUCU
UGAUCCUGCUAUGUCUUUUAGCGGUAUUCGACCAUUUUAUGGAGGGACUAAUAUGGUCCA
AGGUGAAUUAAACUUUUCUAAACCCGCUGACCCUCUUACAUAACAACCAUAUUGCAGGAA
UCGUGAUUGUAGAAAUUUUGCCAAAAUGAUUGUUUGUUCGGGUUUGUCUUUUAGUUUUUG
UGAACAUUCUGCUUUCAUUGAAUAUUUUCUAAUACAUUUAAUCCUAAUUAUGAAGGUAUA
UCACGAAGUAUGGUUAAAAGAG
2. MFE structure-
.((((((((((((.((..(((((((((((((((((((((((((((((......((((((..................((((.((((((((((((.(((...((((((..................(((((...((.....((((((..((((((((((........(((((.(((..(((((((.(((((..........
.....(((((..(((((.....)))))..)))))(((((((((((.....(((((((((.((.(((((((((....)))))))))..))...))))))))).................(((((......)))))(((.(((.((....)).))).)))...............................)))))))))))
........))))).))))))).....((((((((((.((....((((((....))))))))))))))))))......))).)))))................(((((((((((((((.(((((..(((((((.(((........)))...))).))))..))))))))...((((((...))))))..))))))))....
)))).)))))))))).....((((((....)))))).......))))))....)).)))))))))))))).)).)))))))).)).))))............(((((..((.........))..))).))......((((.....(((.(((((..(((((...........)))))..)).))).)))))))...((((
.(((((.........)))))))))((((.((..((.(((.((........)).))).))..))))))...))))))..((((((((...............))))))))..)))))))))))))...........))))..)).)))))))....)))..))..(((((((...((((((..((((..((((((((((((
....((((...((((((..((((..((((((.......))))))...))))((((...))))..((((((...))))))..((((((........)))...))).((.(((((....(((.(((((.(((.((((((((.((.........))..))))).))).))).))))))))..))))).))...))))))..))
)).....)).))))))))))..))))))))))....)))))))....................))))).)))))))......
You can download the minimum free energy (MFE) structure in
II. Results for thermodynamic ensemble prediction
1. The free energy of the thermodynamic ensemble is [-272.63] kcal/mol.
2. The frequency of mfe structure in ensemble 1.84887e-16.
3. The ensemble diversity 295.52.
4. You may look at the dot plot containing the base pair probabilities [below]

III. Graphical output
1. A note on base-pairing probabilities:--
The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure
plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and
violet (high entropy, ill-defined).
2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.