lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-527.30) is given below.

1. Sequence-
AGUUUAUGCUAUGGAUUAGAUAAUCGGAGCUGCAAUAAAACCAAGGAAGGAAAAACGUUC
UCAGGUAUUUUAUGUUUAACUUGGAAUAUGUAAAUGAAAAGUUAUUCCAAUGCUGUCAGG
UUACAAAGUUGAAGAACUUGAAAGAAUGAUUUGAAAGUGUCACCAUUAAAGCAGUUAUAU
UUUUUAUAAAGCACCAUUAGAAAAGCUCGUGUGUAGGACAUUUUUUAUGUUAAUUUAUCC
UUACUGUUCAUUCUUGAAAAAGCUUAAACUUACUAUAAUUUUUCAAAUAAUUAGCCUAAU
CCCCCAUGUCCAUUUGAUUGGUAUUCCUAUUAUGAUCCUGAGAUGGAUAGUGAACCAAUA
AGCUUUCAUGAAGACUAAGCCUAGUUUUUCAUGGGCUCUUAAUUUAUGUAAGUUUCUUGA
AAUCCGGCUCCUGAAAUUGUUGAUAUACAGUUAACAAUAAAUUUAUCUUUUCUAAUGAUU
CAGAUUCUUAAAGCGGGAAACUUUUCAGAUUCUUUCUAUACAGCAAAGGGUGGUCUGAAU
UUCUUUUUCAUUGUGAACUUGCAGUACACUGACUAUAAUGACAGAAGAUUUUGAAGGGAA
GACGGACCAGACAACCCUUUUUUGUCUAGGUCUCUUAAACUUGUCCAUUGCAUUUCCUGG
AAUCCUCUGUAAGUUAUCUGAGUGUUUUUUUUGUCUCGAGUUGGUAUAUAUUGCAUUGAG
ACUGAAUGCUUAUGCAAAUGCUACCGAUUUUGUAUGUGGAUUUUUCUGUUUGGAUGAUUU
CUUGUAUUAAAACUUAACAAUGAUACGUGGGUCAAAGAGUAGAUGCCAGGGGUUGAUUUA
UUUUUACCGAGAUAGAGAGAAACUUUUCUAUCCUAACAAAAUUUCUGUAGAAGUGGGAAG
AGAUACCUGAUACUUGGAUAAAAUGUAAUUUUACUACUAUUCCCUUUAUAAAAAAACUGA
AUCAAGGACACGCUUUAGUUGUUUAUCUUUCGUUUCAUGCUUUCUUGUUGCGAUUUCAGU
CUGGGUUGUCCAUAUAUUUGGUUAGAAAUUGUAUCUGUUCUAUGUUUCAGAAAACAAUGA
UGUUGUAAAGACUUACAUGAUAGCUUUAUCUGUAUUACUGCGAGUUUUUUUUUAUUUUGA
UGUGCACAUAACUCUUUGUUUUUAUAAUGAAGUCAAUUAAUUAUCGAACAGUUCUAAAGA
AGACUAAUAUGUCUUAUCUGUACAUGAUCGACAACAUAACUUUUGUUUCAAUACGAAUGU
UACCUUCUCAAAAGAAAAAGAAUAAUAUGUUUCAGAUUUAUUUUUUCUCCUUUAUCGGUA
GCCUAUUAAGGAAUGUCUCUUGAAAAUUUGUAUGCUAGUUAUCCACAUAAAAAAUUAGAA
AGGUUAUACAUAUUCUUGAUGUCUUUUUGAAGCUGUCUUGUUAUUAAUCUAUAAAAUGCC
AUGUGAAGACCACAUUUUUCUGAUAAGUUUAUCGGUCAUGUUAGAGGAAUAAAAUAAUCA
AAAUUUAUGUUGUCUGUUUACUGUAGACUGGUGUGGUCCUUUCCCUCAAACUUUGAUGUU
GAUCUAAACUGGUUCAUUUCAAACCUUCAGAAUUGAACUAAUUGCUUGUUAAUUUGUUUG
UACAGCCUGCUACAUUUUCUGCUAAUGUAAGAGCUAGUUUCUGCUGGACUAUUUACAUUA
ACCUUUUAUUUCAAAGUUUGUUUCUGCUGGAACUAUUUACAUUAUUUUCAUUUGGCAUGC
AAAGUAUGUAAUUGAAUAUGACCUUUUGGGAGGUUAAUGUAAUUUCAUUGUAACUUAGUU
AUACUUUUAUUUCUUCUACUUUCCGGAGAUUGGAAUUUAUGUAUGGAUCAUUCCAAGAGA
ACUACCUUGAAUUGGUAAUGUAGAAUAUUUAAUGAAAACAUCGUUUAGCUAUUGUUCUCU
AUCAUUUUGCAUUUCCAUUUUUAUGUGGUAUCAUUAGCUGUCUUAUUCCAACUUUUGCGG
GUGAUUAUAUACAUAACUGAAGGUAUUGAAAGAGCCGAGACAGUCUCACUGAGGCUGCUU
CCUUAAGGGAGAGAGUUGCACUUCCUAGUGUAAGUCUAAGGGUUGCUGCUGCAGCUGCUU
CCGUGGUGGAAUGCGUACUGUCAAUUGAACAAAAAAUUGCAGAUAAUUGAUAUAUUGAUU
ACGACAUUGCCCCUGACCAUCGGCCAAUGCAAGCUUGUUCAAAGACUGAACUAUACAAAG
UUGAUUAUCGUCUUUGCUAAGAUUUUAGCCUAAAUCCUAGAGAUCUUAGGAUUUGGAUUG
UUUAAACAAUGCCAUGUGACAACUGAGAUAUAAAUACAUUUGCU

2. MFE structure-
.......((.(((((((....))))((((.((((((((((((..((..((((.(((.(((....((((((((.((...)).))))))))......)))..))).))))....))....))))...(((((....)))))(((((.((((..((((((....((.((((((((..(((((.....))))).((((.((.((
.....)).))))))..(((((.....)))))......(((((...(((((...((((((((.................))))))))...(((((.((..((((.(((((((((((....((.(((........)))))..))))))))).))...(((.((((((.((((((.....((((((........))))))...
..)))))).)))))).)))(((((.((((((((..(((((((((.....))))))))).....(((((.((((..(((((((...((((((.((((((((...(((((((....((((((..((((((((((((..(((.......)))(((((......)))))(((((....(((((.(((((.((((((..((((((
((((((((((((((.......)))))).))))).........))).......)))))))))))).)))))..)))))...)))))...(((.(((((((((...........)).))))))).)))(((....)))...))))))..)))))).)))))).......)))))))...)))))))).))))..........
.))....))))))).)))).))))).)))))))))))))((((((((.(((((((((((....)))))))))...........)).))))))))))))..))....)))))....(((......(((....)))......)))..............))))).)))))...)))))))).))....))))))..)))).)
)))))))))))).)))).((((.((((((((((.....(((((((..(((((.((((..........))))..)))))((((((((((.....((((.((((....))))))))...((((((.(((((.((((..(((((((((........(((((((.....)))))))...........((((.((.........(
((((......))))).........)).)))).....((((.(((((......))))).))))..((((....))))(((((((((............)))))))))((((((((..((((((((.....(((.((((((..((.((((........)))).))(((((......((((((((..(((.((......))))
).))))))))(((......)))..................)))))..(((((((((.........((((((.((((.....((((.(((((((..........))))).)).))))...))))))))))))))))))).......((((...)))).(((......))).(((((.((((((((((((((...((...((
(((((((((....(((.....(((((.((((((((....((..(((((((((.(((((....))))).....(((((((((((((..............(((((....)))))........((((.((((....((((((...))))))...)))).))))((....)))))))))))))))......((((((...)))
))).(((((..(((((........))))).)))))........((((....))))(((((((((((.......))))..((((.......(((((.....)))))...)))).)))))))....)))))))))..)).....))))))))......))))).......))).....)))))))))))....))...))))
)))).))))))))))))))))).))).....))))))))....))))))))(((.(((((((....))))))).)))...((((((....))))))((((((.....)))).)))))).))))).)))).))))).))))))................))))))))))....)))))))..(((((((........((((
(.....)))))........))))............(((((....)))))(((((((((((.....)))))))))))(((((....))))))))))).))))))).)))).......)))..)).

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-571.80] kcal/mol.

2. The frequency of mfe structure in ensemble 4.37257e-32.

3. The ensemble diversity 657.25.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS