lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-305.30) is given below.

1. Sequence-
CUUUUAACCUGCUGCUUAUAUGAUUUUUUUUUCUCUUUGUUGUCUUUGCAAAUCUAUUCA
UACUUGAUGAUCUAGCUUACCUGUAUUAUUUGGUUACUGGAUGAUAAGGAAGUAAUGGGA
GCAUGCUAGUGUUAACUUGUCUAUAAAAGAUGAGAAUAUGGGUAGUAAAUCGUAUUAGAG
AAUGUUGAUAGCAUGAUUUCCAUCUUACUAAAUCACCAAUAUAUGAUUGCUUUGGAUUGA
UCAUGUCAACUUUGCUGAGAAUUUUCCUUACUCUAGCUAUGUUCUGCCUUGUAUGUUGUG
CCUAGAUUUACCUUCUAAUUGAUUAUAACUUUAGUUUUAUAUGGUUAGUUUGAAGUCUAU
CUUGUCCCUUUAAUGCUUGUUGUUUGCUCACAUGAAAUGGAGCUUGUGGUUAGUCAAUUU
GGUUGUGUAUUACACUAUCCUUGAAUCCUCAGUUCACAAUAUCGUAACUGAACCUACCUC
AAUCCUUCUCCCAUACAUGCUGCCCUAAUAAUAGAAUGUUCAUGUUCAUUAUUCAAUUUA
UCUAACCUUAUCUAUCAGAUCUUCCUCUCUAUUGCAUAUAUGUGAUCCUAUUGAGUUAUG
UUGAGUAUAUUGGUCUUGUAGAUAAUGUACUCUUAUUUGACUGGAAUAUGCAUGCUUGUU
AAGUUAUGCUUAAACCUAUCAAAGAAAUACCAACACUUUUUAUGAUCAGUGACCUUAACU
GUAAUCUUCUAUACAUAGGUGUUAUGAGUUCAACAUAAAUUUUACUAUGUAUCUUAGUCC
AAUAUGUAUGCUUGGUCGAAACUAUUGCUUUAAAAUCAUAAGUUAAAAAACCAUGUUAAU
AUCCUGCAAGUCUAGUGUGAUAUAACCUAUUACUAAUAUUCUCUUCCCCAUGAAUAAAUC
AAUAAUGUGAUGGUUUUGUUCAAUAAAAAUAGAAAUUAAGAUGUAUGGAUCAAGUGAGAU
UUCUGUGUAUUGUUAUCUUGAAUCAUUUACUAGGGUAUUCAUGUAGCUAAUAUGGUAUUA
UUUUCUCAAGAAUGCAUGUCCCGUUAUAUUAAUACAGCUUUAGUUAUGAUUUAUAUAUUU
UGAAAUUUAAUACGACUAAAUCAGUAUGUAUGGGCCUCGACUUGAAGCUUCAUGGGCCAC
UUGGCCAGUUUAUAAAUCCAUGUGCAUUAUUUCAAGUUUUAAGAAUGGUGUCUUGAUUUU
CUUGUUGGGCCCUAGUCUGGUUUAAUUUUUUGUUAAUGAAUGUUUUUUCAUUAUCUAUUU
CCUAUUUUUUUUUUGAUUUCUAAUGGGUUCACCUUGUUUUUCAUAUCCUUUGUGACCUCG
CAUAUGCCUGGUGGGACCAAAGCCCCUUUUAUAUGUUGACCUUGCGAGAUUUGGGCCCCG
GCUAGCCCAAGGAGUCCUUUUUUCUUUGUUAUAUUUACCUCAAUUAGAAUUUACAUUAUU
CGCAUACGUUUUUUGGAAUCAAGGUGUAAUCCCGGUGCACGAG

2. MFE structure-
...((((((((((((((.(((.((((((((.((..(((((.......))))).....(((....)))..((((((...(((..........)))..)))))))).)))))))).))).))))).)).)).)))))(((((((.....))))))).((((((((((.....((((((((((...((((...((((((((..
.((((.....(((((........))))).....)))).)))))))))))).....((((.......)))))))))).))))..))))).))))).((((((((.((((((((((((((((((.(((((........))))).((((((((.((((((........((.(((((.(((((..((.(((..(((((..((((
(((((..((((((((((...(((.((((...)))).))).))))....((((((.((......))))))))..................(((((((((((((....((((((.((((......)))))))))).............(((((((((...(((((....((((((((((((((((......((((((.....
..((((((((((.(((...)))))))))))))...(((((..((..((.((((((((...))).))))).))..))..)))))....................(((((((.......))))..)))....((((((((...(((((.......)))))......))))))))))))))...))))))))))((((.((((
((((((((.(((......................((((.((((.(((.......))).))))))))..........(((((..........)))))......))).)))))))))))).))))....))))))....)))))..)))))).)))..(((((((.((((((.........(((((((..(((((((((((.
(((((((..(((((.((((...........)))))))))...))))))).)))...)))))))).)))))))))))))...))))))).............))))))))))))).)).))))..))))))))).((((....))))............))))))))..))..))))).)))))...))......))))))
......((((((.(((((.((............(((((((......))))))).......((((((...............)))))).............(((((.....)))))..(((((((((...((.((........))))...)))))).)))....)).))))).)))))).))))))))(((((((......
.)))))))...........))))))))).........((((.((.........))))))..))))).))))..)).)))))).

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-334.79] kcal/mol.

2. The frequency of mfe structure in ensemble 1.66686e-21.

3. The ensemble diversity 337.41.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS