lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-379.90) is given below.

1. Sequence-
AUUCAGUAUUUUUAUCUUUCUUUAAUUAAUAACCAAAUACACGAGAUAGGGAGCAGGGAG
AUAAAUAUUCUGCCUUCCUUCAAAAUCACAGUUUUUGAGAAGAGGAAGCUCUCUCGCUAU
AGCACUUUGACAUAAAUUCUCUAAAAGAAUUUGGUAGCACCAACUAGCCAACAGAUACAU
UUUAAUUACAUAUAUAUGUAUAUACCUACCUUCAUCUUUUCUCUAGUAUUGAUCCCUUAA
AUUCCAUCCAUCUCCUAAUAAAAGACGUUCGUUCUUGUAAGAUUAGUAAUAUGAAUAUUC
UUGUACUUUUACGAAUUCAUUGCACAAAAAAAAAAAAAGGAUAACGUGCAGCUUCAAAUU
GUGAACGAUAUCCUUUAUGAAAAUGGCAAAUCUUUCGGCAAGGCCUAGGAUUCUCAUAGA
CAUCCUGUCCUGAGAGCCCAUUCACCUUAGAUUUUUCAACAGAUCUUCCUAAACCCUUUA
GGCGGCAUCUUCCCUUUGAUUAUGACGAUAACUUUAAUUAAUUGUCAAGAUAUAUACUUA
UAUAUACGUAUAACUUUUGUAUCUUAGGAGCUGAACACAGGCUCCUCCCAUAUCCUGGAU
CCUAAGAUUCAACUAACGCUGAAUCGAUCUGCACGGGUGUCAUGCGCCCCAACAGUAGUC
GAGCAAAUAUAUUAGAGACCUUUACCAUUCCAGCUAAAUUUUAUCCAAGUCAUCAAAGUG
UGUGAGCUUUCAUUAUGACGGACUAACUUCCAAAAAAUCUGGCCAUCUUAAAGCUUUGGG
GGACCUUCAGGUAGAGCUGCUGUUGUGAAACAUGCCAUCAAGAUUAAAGCACACCUCGUA
GUAGCUUCUCCCAAGAUAAAAGCACACUUCAAGACUUUUGUAGUCUAAGAAAAUGUUCAU
CUUCUCCUUUGUAGUAUGACAUAAACUCUCCUGCUUGGCUGCACUAACACUGUCCAUAUU
CUCAGACUUCACAGCUACGAUUCUAUCUCAAGCAUCAACCAAGCCAAAGUUCUAAGUUAA
UGCUAGCUUCCUACGACAAUACUCUCUUCCCCUUAACAAAUGGCUAAAAUCAAAUGAUAA
UACAUCAUUCCCCCCACUACAACAUGAAAAACUUAAUCCACUACUGCUAAGGGAAAACAU
AAUAUGCUACUCAAACAGGAAGUAUUUAUACAACCCGCCUGUGUCACCCCCUAGGCUUCU
CUUCUCAACUGUUUAAUGAGCUAAAUAAUCUCUCUUGGUGCUGAAAUCUAGCUCUAAAGA
AUAACCAAGUCGAGCCAACAAGGUAACAUUUAAUACAGCUAUAAUAUGAGGAUAAGCAAA
UUUCUAAAACUCAGCCCAGAACAGCCCAUUGCACUACAAGAAUCUUUUCCUGUCACAAGA
CAUCUGGAAAACAUAUCAGAAUGAAAUAACAAAGAAUUAAUCUGGAGACGUGUGAACGUA
AUAGUCAUACUAUCAAGUCACAUACCCUGAGUACCUUCACAUCUUGAAGAUGCAGCAUCU
UCUGGAGAGAAAUAGCAAAGGCAAGCUCAGCCUGCUGUCUGAUAUUCACUCAUAGUUAAA
UUAGUUUAAAAAAACAAAGAGAGAAACCAAAAUUGAUAAGCAAUGAAUGACAGUCGAGAA
AAAAACACAACACAAACUGUCAGUGAUCAGCCAUCAAGAAAAUCCAACAAACUAACAAAA
UAAGUUUGAAAUUAUGUGCUAGUCAGCUAUGACAAAAGAUAAACGUUUGGCACUAUUAAU
AUCCAUAAGGCACGUCAUUAGCUGCUCCAACACAAUAGGAAUGGACUGAACUCCUCAUCU
CUCUGCAAAUAUCAUAAUAGUACAGCAAGGUUAAAGAUGUAAGUCCGAAACUUCCAACAA
GUAAGACAUGGCAAGAGGUAAGAAAUCGCGUGAAGUGCUCCAACAAAUCAGCCUUGUACU
ACAAGCAAAUAAAAAGUUAACUGCAUCAUCAUUUCAUCUCUGACAUGAACUUU

2. MFE structure-
...((((.(((((((((((..............((((......((..((((((((((.(((.......))).)))((((((((((.......))))))....)))).)))))))..)).......)))).....((((((.....))))))((((...))))....((((....((((((...............)))))
)........(((((((((((((((((.((((((..(((................((......)).((((((((((((((......(((..((((((....(((((....))))))))))))))............((((((((.(((...((........))..))).)))))))).....((((((...(((...(((.
(((((((((.....(((((((.((((((..(((((((...(((.((((((.........((((((..((((((...))))))....(((........)))...((((((...........))))))((((((((...((((((....))))))....))))))))(((((((.......))))))).(((.....)))..
.....((((((........))))))))))))...((((((...))))))...(((((((.((((..(((((((.(((((.............................))).))..)))))))..))))..........(((......)))........(((((((......((((.((..((.(((.((((((((.((.
.((((((((.................((((((........)).)))).....(((((...((((...(((.(((((.....)))))..)))..)))).)))))....((((.(((((((((..........(((......))).......))).))))))..)))).))))))))..)).)))))))).)))..))..))
))))....((...((((((....))))))...)).........................)))))))......((((((.....))))))..............................))))))))))))))))..........))).)))).)))))).))...............)))))......)))))))))..
........((((..((((.(((.......(((.((((((((((.....))))...........))))))..))).......)))..))))....))))........((((...................))))))).)))...)).))))....))))))))).((((((((((....))))...)))))).......))
)))........))).))))))))))))).(((((.((...(((((...)))))...)))))))...((((((.((((....(((.(((((((...))))))).)))...........))))...))))))((((((((((((((((.................((((....))))..(((((((.......((((.....
))))...(((((((.....................)))))))....((((((((........(((..((((((.........))))))......((((..((.((((((((((...((((....)))).((..(((........)))..))..)))).))))))))....))))...))))))).))))........)))
))))...)))))).....)).))))).)))..))))))).)))......((((.....))))......)))).)))))))))))...((.((.(((((.................))))).)).)).............)))).....((((.(((....))).)))).....

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-419.70] kcal/mol.

2. The frequency of mfe structure in ensemble 8.95861e-29.

3. The ensemble diversity 652.03.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS