lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-369.80) is given below.

1. Sequence-
GAAAAAUAUAGUGACAAUAUUAAAGGUUAAACUGAAAAAGGUAAAACAAGAUUUUAGAUG
CUUCUCAAAUUUCACAAAAAAAUAGAUUUUAGGUAUUUUUCUGAUUUUAAAUACAACUUC
AAGUAGUAUUUGAAAUUCUCAUGACUAAAUUCUAAUUUUCAAAAGAAGUAAAAAUAAAAU
UUUUGACAAAAAAGCGAAAAUCAUGACCAACCCGGUCUUUAACAAUUUUGAAUAUUUAAU
GUUCUAAUAAUCCUUAUAUGAGUGUUAAAGUUUCAUAAAAACUGAUGUAUUAAUGGAAAC
UUAAUAGAGAAACUAUAAAUUGAGGAUGGAUGAGUAUUUUGUACUACAUCUAUCCAAUUG
UGUAUGGUACAUUCGCCUUAAGAAGUUUCUAACAAGAAUUUCUCAAUGAAAUUUAUUUUC
AUUUAAUAAAUUUUAAAAAAGGGUAUGAAAUGUGAAGUUAAAUAUUUAAUAAAACUUACG
GUCCGUUUAGCCGAGAUAAAAUCAUGAUUUGAAGUCACGAUAUAAAAUCAGUUGAGAUGG
AAUUGAAGUUUUAUUUGGAUAUACAAUUUUAAAUUUGUAAGUUGUAUUUUUUUAUAUCAG
UUGAAAUGACAUAAAAAGUACUAUAAAUCAAAAUAAUUAAUAAUUUAAAAUAUUUAAAAG
ACAUAUAAAAGAUUCGGUUGACCCUAGAAAUUUCUAUUGGUGCCACAUAAAUUGAGACAA
AGUGAGCAACAUAUAUUAUUUGAAAAUGACAUAAAAAGUACUAUAAAUCACAUUAAUUAG
CAACUUAAAAUAUUUAAAAGAUAUAAACAUUUGAUUGAGUCUCGAACUUCUAUCGUUGCU
ACAUAAAUUGGGUUAGAGAAAGUAACAAUAUAAUUUGAAAAUGACGUAAAAUGUAAUACA
AAUAACAAUAAUUAACAACUCAAAUAUUUUAAAAAAAUAUAAAAUAAUUUGGCUCUCGAA
AUUCUUUCAAUGCCACAAGAUGGAGAAAGUAACAUAAGUAUUAUAAAUCACAAGAAUUAA
CAACUUCAAAUAUUUAAAUAACAUAUAUAAAAUUUAGUUGACUAUUGAAAUUCUAUAUGU
ACCACAGAAAUUAAAAUAAAAAAGGACAAAUAUACCCCUAAACUACGUAUAUACCCUCCG
UUAUACUUUAGGGACUUAUACCCAAUUAUCAUACUUUAAAGUCACAUAUACUCUUAUUCC
AAAUGGAGUGACACAUGUUAAGAUCUGAAUUGUUUACUCAUUUUCUUUUAUUUUUCUAUU
CAUAAUUUAAAAAACGCAUUCAAUUAUUACAUUUAACAAAUUACAUACUACUAAACCUGU
CAACUGGGCCGGUUUACUCGAAAAAAUCGGCCCAAAAUGACCGGACUGGUCCGGAACUUU
UUUUAAAAUUUUUUUUUAUUUAAUUUACAAAAUUAGUCGUUUUUAGCCGUUGGCCAACAG
UCAAAAAAGGCUAAAAAGAGCUAUUUAUUAAAAAUAAUUAUUCCAAUUUUUUUUCUGUAA
AUACCACUUCAAAAUUUUCAUUUUUCACAACAAUUCUCUCAAUAUCUAUCAAUUUCUCUU
AAUAUUUCCUCUAAAAAAGUGUGUGAAUUCUUAAUUUAUUAUUUAGCAAUUAUUAACAGC
AUUAAGUGAUUAAAUGAAAGUUAUCAACUUUCAAGUGUUCAUUUAAUAUUGGCAAUUUGG
UAUAAUCGUUCCAUCUCUUAUUUUUAUUUUUUGAGUUUUAUAUUUAUUUAUUAUUUACAA
UCAUAUUAUUUGUGUGCUUUAAAUUUUACCAUUAUAAUUUUUAAUAUGGAUAGACUAAGG
AAAAUGGGUAAAAGUGUUAAAAAUUUGUGUCUCGGAAGUAGUCUUAGUAAUAAAAACACA
GGUUUUGCUUUCGCUAGAGGUUCCGUAGUAGUAGGAAUUAUAAUCGUAUGUCUGUCGUUC
CACUUAGUAUUGCUAGUGAACCCGAACAAGAAUAUGAAUACGGUUGGGGUGGGGGUGGGG
GUGCCGAUGAUUUUAAUUAAACGGAGUUUCAAAAAAGUUACGGAAUAAAAAAAGAUAAUA
AAUUAGAAGGGGAUAUUAAUAUUAAUCCUGAUGGA

2. MFE structure-
(((((....((((............(((..(((......)))..))).((((((.(((....))).)))))).....((((.((((.(((((..((......((((((((((((.(((...))).))))))))))))...))..))))).)))).))))......(((((((((.((((((((....(((((((......
((..(((((..((((((............((((((...))))))(((((((.......(((((((..(((((((......((....)).......))))))).((((.....)))).......((((((((((((((...))))).)))))))))...((((((((((((....(((...((((((((((....((((((
(...(((((((.....)))))))....))))))).....(((((.(((((((((...((((((((((((((((((((.(((((((((((((.((......(((.(((((...))))).)))......)).))).)))))).))))))))))))))..(((.(((((........))))).)))(((((((((.((.(((.
......))).)))))))))))..........................))))))))))....)))))......))))....)))))))))))))))...)))(((((...............))).))....(((.(((((....))))).)))....))))))))...)))).....(((((((.....((((((...))
))))........(((.((((.....))))..))))))))))..(((((((((.((((((((...............((((((((.(((((............(((((.................(((((((........)))))))...((((....((((...))))...))))...(((((((.........((((((
((..........................))))))))....(((((((.....((((((.....))))))....))))))).............................((((((...........))))))...)))))))..(((((((((....(((......)))....)))))))))...........(((((((
....)))))))....)))))............))))))))))))).....)))))))).)).)))))))......))))))))))))))...................................(((((((((((........)))))))))))....))))))..)))))..)).))))))).))))))))))))))))
).(((((((....((((..(((((((((.(((((.....)))))....)))))))))..))))......(((((.((((.....)))).))))).)))))))..))))......))))).........................((((((((((....((((((((((((..................(((.((((((((
(((((.(((..(((..(((.(((((((((.((..((((((((...))))))))..)).))))))))).)))..)))))).))......((((((((((.............(((((..((........))..)))))....(((((.....)))))..........(((.(((((((((((((..........((((.((
((....(((.(((((....(((((((((((((.(((.....))).))........)))))))))))))))).))).....)))).))))..))))))))))))).)))............((((.(((...))))))).((((.(((...............))).))))))))))))))...(((.(((((((....((
((...))))....))))))))))..........))))))))))))))))))))))))))....)))..)))))))

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-413.58] kcal/mol.

2. The frequency of mfe structure in ensemble 1.4222e-31.

3. The ensemble diversity 537.73.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS