lncRNA Structure Prediction

Species Name : Withania somnifera Sequence Id : GBHJ01061898.1

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-338.40) is given below.

1. Sequence-
ACAAGAUCUCGUUCUAGAUCUGUCUCUUCUCAACCCCGUUCUGGCUUAAAAGGACGCUCU
GUGUCAAGGUAAUCUUCCUUACUAGUCUAUCACACACACUCUGUAUAUAUAUAUAUAUAU
AUAUAUAUACACACACACACUUAUUGAAAGUCCUUAUAGUAGUCUAUCAGUUUAUUUGAA
UCAUUUUAAUCUUUCCUUGUGAGAGGUACCUGUGAAUUAACUGUUGCAUCUUGCACUUCA
UUCAGUUAGUGGGCAAGUGCUGAACUCCUUGUUUCUAACAUCCUGAAUCUUGGCUAGACU
UUGUAUUAUUGUUGACUUGAGAUGGUGGUAAGCGAAAGUUAUUUUGCAAUGUGACUGAUU
UCACAUUCACACAUAUCUCUGCUUGCAAAUUUGACAAGUUCAAAUUGCAAACGUCAUCUA
AAAAGUGAUUUUGAUUGUUCCCCACCUCUUCCUUUCUAUUUAGAAAGUGAAAGUCCUUGC
CGGUGUGACAGUAUGAGUAGACGGUGCUCUUUUUUGAUUUAUUUGAGAAUUAGUUGUAUA
UUAUUUACAAUAUAUCUGAGGGGUUUUGGCCUCACAACAGGCUUCUUAUUACCGUAUAUA
AAUAAGUCAUUUUUUUCCGCAUACGAACAUUAUGCUUCUAAUCCAUCAUUCAGUUUGAUA
GAUAUAACUUUUCUAAAAGUAUGUGAACCGUUUGGAAAUUUGUCAAGGUCCUAGAUCAAU
GUGCAAUUGUUCAGUGGGUGACUGGCAAGUUGUCAUUAGCAAAUAUCCUGCUCUUAUUGU
AGUUUUAAGAGGACUUACAUUAUGGUAAGGUAUAAUUAUUCUUCUUUUUUGCGGUGGCAU
AGAGAUACCUAUUGCUAGUUCCAUUCUGCUUCAGGAUGUCUUUAGUGCAAAGAAUAGUUC
UGCUUCAGGGUGAUGUAUAUUUUUGAACUUGAAAGCAAUGUCAACCCAUUUGAUAGAACA
AGCAAGUUACGGAACAUCAUAGUUUUCUGCACCUUUUAAGAGUCCUGUACUUCUGUUCAU
GGUGCAUAGGAUUGUUGGUUGUUUUUUUAACCAUGCUCUUCAGAUUUUCCAACUGUCAUU
GCAACUUCUGUUAUUAAUUUUGUGGUUAACUGGUCAUUCAGUUACGAAAAGAUGUCUUUG
UACUGCAUUUCAGAAUCCAUUAGAAACACAAUAGUUGUUGGGUGUUGGAUUUAAAAUUUG
GUUGGUCCUAUGCUGCUUGUUACUUUUGCAUUUUGGAAACAUCGGAAAAUAUCCUGUGUU
GUCAAGGUAUAAUCAUAUAUUAUUUCCUUUGUAAAUAUGAGAAAUCACAUUGUUGGUUUU
UCUAGAUGUUGGAUAUAUACGAGGAUCUUGGAAUGCAUAUUAGGAUCUUGAGUGGAUAUG
GUCUUGCAUACGACAAAUUUUGUUGUGGAG

2. MFE structure-
(((((((.((((...((((((((((...(((((...((((((........))))))...(((((...((((..((........))..))))..)))))...(((((((((((((....)))))))))))))..................(((((.((((((.((((.((((.....(((.((((....(((((((.....
)))))))....)))).)))))))..(((.(((((...(((((.....))))))))))))).....(((((((.(((((((((..((.....(((((..((((((((((..........(((((((((....((((((....))))))(((((((......)))))))..))).))))))...((((((.(((((.....)
))))))))))..(((((((((((((.((...(((((((.((..(((...((((((((....)))))).))((((((((.........((((((.((((((....((((......((((((......))))))((((((.......))))))...((((((.(((((.(((((......))))).((((((..........
))))))............(((((.......))))).....(((.((((.......)))).)))...(((((....)))))....))))).))))))(((((((((....((((((.((((.....)))).))..))))....)))))))))......))))......)))))).))))))........))))))))....
...)))..)).)))))))....))))))))).))))))...(((((((((...((.((.......))))...))).))))))))))))))))..))))).(((((((((..................)))).))))).((((((.....)))))).((((((((.((...(((.((..((((((((.((((((.....((
((........))))......)))))).))))))))((((((......))))))......((((((((((((((..(((..((((((..(((......(((.((((....((((..((.((((.(((((........))))))))).)).))))...)))).)))...)))..)))))))))..))))))....)))))))
).)).)))...))))))))))........(((((((.(((((.(((.....))).))))).)))))))....((((((.((((.......))))))))))(((((((......)))))))))..))))))))).....)))))))...)))).))).))).))))).))))).))))).))))).....))))...))))
))).......

You can download the minimum free energy (MFE) structure in

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-365.66] kcal/mol.

2. The frequency of mfe structure in ensemble 6.16023e-20.

3. The ensemble diversity 383.22.

4. You may look at the dot plot containing the base pair probabilities [below]



Download Dot plot

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot

* Results have been computed using RNAfold ViennaRNA-2.5.0.
* An equivalent command line call would have been RNAfold -p -d2 --noLP < test_sequenc.fa > test_sequenc.out
* A dot plot into the mountain plot coordinates transform using mountain.pl test_sequenc.fa | xmgrace -pipe