lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-371.20) is given below.

1. Sequence-
CCUACAUUUAGAUACUGUCGUCAUUUGCUUGUAUUACAUCCCAAAUCUUAGGCAAGACAU
UGCGUCUAAAUGUUUGUAUUUACAAAUACUAGAUUAAUACAUCAAAGAGAUACAUAAAAG
AAAAUUACAAAAGAUCAAGGAUCCUGGCCUACGAAAAAACUGGACCAUACAAGGAGCGCA
AUUUACCGCAACUGUUGUAGCUCCAGGCAAGAAUCAAGUCGAUCAACCACCUAGAACAGG
UUCCAAACCUAUGUACCCUCAAAAAAGGAGGGAUUUUUAAAUAAACUGCACGGGGGAGGG
GAAAAAAAGCAACACGACAAAUUAUAGAGUUCUCUAUUUUCUAGAAACAGGAGCAACUUC
UAAAUCGGCAUGAUUAGUUGUAGGAAAUCAACAUGUCCUUCUAAGCUUAACUUGGUGUAA
AUACUUGCAAGAGAUCAACCUCUCAUACCCCAUGAUUUGAGGAAACCACUCUUCAAAACA
CAGCCACCCUCCACCAAUCUCGGUAAAGAAAGAACAAACAAUGGAGUAUUGCAUACGAGG
AGCAAUAAUAAAAGGCAUUAGCAAAAGAAGUAAAUACUGACUUAUCCAACAGAACAAAGA
AGAGAUGACUUAACUUCAGAUAGGAGCAGAGAUUUUGAAACAACAUUCUCAUCAAACAAA
CAUAGAAAAUACGAAUACCUAUUUUUUAAUAUACAAGUAAAUUCCACUAAACAUACUGCU
GAUUCAUUGCACUAAAUAUCAGAAGCUCGCAGAUAAGUCAUAUAACAAAUGCAUUUUUAG
UUUGAUAGGUGAAUCUUAUAACAAAAUUAAGCAUGUUAUAAUAAGAAUACAAGACUUACC
AUAUGAUUUUAACCUGCUUCAAUUAAUUCCAACCAGUACUCCCCAAAAGCAAUCAAAUUC
CGCAGUGAAAAACAGUGAAAUUAUAUCACUGCAUUUUGACAUACUGAUACAUCUUGUACU
ACUAAACCACAUAGAAAAAUGCAUUGAUGAUCCAGUGUCUACAGAGAACUGUAGGCAACC
AUGUAGUUUAAGCCGUAUGCACAUUCCAUAGCCACUACAUCCUCUUCAAAAGAGAGCAAU
CUACUUUUAGCAAUAACUUUUGCCAAUAAAAUGACUAGGAUAUUCAGAAAUUCACGAUUG
AAUAUUAUCACAGAUAAUGAAGAAACAUGUGGGCUUUCUAGUGAUUAACAUAGCAACACU
UUAGAAGAUAUUUUGAAAGGUCUAGUAUCCCAACAUUGCAUCAUAAUCAAAACUAGAAAG
ACACUACGAAAUCUGCACCAGGGAUAGCAUCUAGAAGCAUCAAAUAGACAAGUGCAACUA
ACAGUAGAUUCAUACAAAUAACAAAUAACCAGAAAAAAAAGACAUCAGGACACAUGGCCG
AGAUUUAAGAGUUGAAUUCAUCUAUAGUACAACUACAUAAGGGAAGAAAGGAUGAGGCCG
CUGGCUACACAGGUUCUUGGAUGCACAAGAUUGGAAGUAUAUGGUGUCAGGGCUGGCAAA
UUUCGAUAGUAGGUAAGAUCUUCGGAUAUGGUCCUGCUAAGGAAAACAUCAAGGUAGGCA
AUGAUUUUCGAUGUCUGCAUUCAUCUGGUCAUCUGGUUUAGGCAUGAGGCUUCAUCAAAG
GUGACAGUAGCCAGGCUUCAGGCUUCAGAUAUCUCGGCUGUGGUCCUGUUACAAGCUCUA
GUAACCAUAACUUUUUCUCCGGGAAGCAGAAUUUUGCCUUGAUGACC

2. MFE structure-
................(((((((((((..(((((((...............((((....))))(((((..(((((((....))))))).)))))))))))).))))..........................((((...)))).(((((............)).))).....((((((((((...........))))).)
))))(((((((((((.....)))..............(((((...))))).....(((((.......))))).............((((.(((((((((((.......................(((((((((((..(((....)))..)))).))).))))((((.((.((.(((((((((...............(((
(((.(((...(((((((((...........((((......))))...(((((((.((((((((......)))))))).....((...((((...(((((((.((................)).))).)))).....)))).))...........((....))..............((((..(((((...........((
((((((((((((.((........((((.((((..............))))............(((((((((........))))))))).......((((..............)))).(((((..(((......))))))))..))))..)).))))))))......(((((((((((....((...(((.....(((((
(((..........))))))))......(((((((..((.((((((.........(((((...........................))))).(((((....(((((((.................)))))))..))))))))).)).))..))))))).......))).))...)))))))))))..........(((((
((((....)))))))))...(((((((....((..((((.......)))))).))))))).))))))((((((((....))).))))).((((......))))..............))))).))))..(((((....)))))((((((...)))))).......)))).)))((((((((((((((..((.((((...(
((((((....))))))).((........)).....))))))..)))))...))))))))).............)))))))))...)))...))))))...............))))))))))))).))))....................................(((((.((((((((((((.....(((((.(((..
.....))).)))))........((((......(((((((..(((((((.((((....((((((((....(((((((((......((((...(((......((((..(((((((....(((....))).....)))))))..)))).......))).))))...)))))))))...))))))))))))(((((((.....(
(((.....))))......))))))).))))))))))))))..))))....)))))))))))))))))(((((........))))).....)))))))))))....))))..))))))))))))))).

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-398.17] kcal/mol.

2. The frequency of mfe structure in ensemble 9.8486e-20.

3. The ensemble diversity 366.06.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS