lncRNA Structure Prediction

I. Result for minimum free energy prediction

The optimal secondary structure in dot-bracket notation with a minimum free energy of (-330.90) is given below.

1. Sequence-
GGCGUCUACCUUUAUGGCGUAUGGGUAAGGUCUGCAUACACCCAAUCCUCCUCAGACCCC
ACUUGUCAAAUAACACUAGGUAUGUUAUUGUUGUUGUAACAUAAAAGCAUUCAAUUGUUA
AAUAAAAAAUAGUAUCUCAUGAUAUAGCCUGUGGAUUCAAUUAUGUAUGUUUAACAGAUU
AUCUCAAGUGUAAAUUAAUAUCUCAAGAUACAUACCGCACAUGUGUAAUUUUUUUGCCAC
CAUCACGUGACUUUGAACAUGAGAAAGUAUAAGUUACAGAUUUUCUUCAGGGUACUGACA
AACUAUGUGAUUCAGUAGCCUACUGUAUGAUAUACUAUUCAAAUCUAUAAAGACAAACAA
GUCCUAGAAGCCAUUCACGCUGUUUGGUAAAAGUCAAGGAAAGUAGACAUCUAUUUCCUC
UAUCUACUAGAAUUUUGGGGACGGUUAAAAAUACUACACGAGGUGAGGAAUAGACCUUGC
ACUGCUUACUAACCCUCUUUCUUUCCCAAGGUGGUGUAAGGUCUGCGUACACACUACCCU
GCCAAAACCCCACUUAUGAAAUUAUACUGACAUGUUAUUGUUGUUGAAACAUAGCAUAAG
CCAAUUCCUAAAGAAAGAAUGGAACUCAUGUCAGCACUAAAUUAGGUAAUGUCUACAACC
CAUUAGCAAUCAACUGGAGUGCCAAAUGCAAAGGGGGGGAAAGAUAAAAAAUAAUAAGAG
AACAUAAACACAGAUCAAAGCGUGUUCAAGUAGGUCGAAGGAGAACAUAUAAUGGAUAUC
AAAAGCAAGCUAGAAUACUAUGACUAGGACAACGACAAACACCAAAACAAAAUCCUCUAA
AAGCGUGACAACACUCCACUACCUACUAACCUUCUACCCUACUACGCAUCCUCUGCUCUC
UAAUUCACAAAAAAAAAAAAAAGCAAGCUUGGUUUUGAGAAAGCAAGCUUGGUUUCGAGA
GAAACCAAACAGAUGAUGACCCAUUAAUCCUAACCACUCAAAUCACACAGAUAAUGGUUA
AAAGUUAAUCAGAUCAAUCAUUUUAUAAGCUCUCCAAGCACCAUAGCCAAUACCUUAUAA
GGAUAUAUUCACAUCAAGAAUCAAUCAGUCGCUUCGAUAUGAAGGAAACAGAAAACAACC
CAUUAAUACUAACCAUUCAAAUGCCAGUGAUAAUGAUACAACUAAAUAAAUCAAUCAAAA
UGACCCCCUCACAAAGCAUUAACCUAUACCAUAAAAUAUAAAAUUCAUGACAAGAAUCAA
ACUUUGCUCCCUUACAAACUAAACAUACAUGAGCUAUUAAUUCUAACAAUUCAAAUCACU
UAGAUCAAUGUCAAAAAUUAAUCAAAUCAAUCAUUUUAUAAGCCCUCCAAGCACCAUAAA
CCAAUAUCUAAUAAGAUAACUUUACAUCAAAAUUCUAUCCAUCGCCUCAAUAUGAAGGAA
AAGGAAAAAAACUCAUUAAUCCAAACAACAUAAAUCACACAUAAAAAUGUAAUCUUGUCA
CUAAGAAGCAAUAAAAUCAAAUACCCAUUAAUCUAAACAAUAUGAAUUAGACAGAAAAAU
GUAAUCUUGUAACUAAGAAGCAAUAAAAUCAAUACCAAGUAAAAUUCAUCUCAAGUAACA
ACAAUAGAGUCGGUCUAAUCCCACUUCUGGGGAAGGAAGAGUAUAUGCAGAGCUUACUCC
UACCUCGUAGAUAUAGAGAGUUUGUUUUCGGUAGACCUCAACCUAAACAUACACGACCCA
UUAAUCCUAAUCAUUUAAAAAACACAGAUCAUGUUUAAAAAAUUAAUCAAAUCAAUCAUU
UUAUAAGCUCACCAAGCACCAUAAACGAAUACCUAAAAAGAUAAAAUUUAUAUCAAAAUU
CAAUCCCUCUUCAACGUGAAGGAAAUAGGAAAGAACCCGAAAUCCAAACAACAUAAAUCA
GACAUAAAAAUGUAAUCUU

2. MFE structure-
....((((..(((((((.(((.(((((((.((((((((.((....((((((((((.....(((((.........(((.(((((....((((((((.............(((((((((((............(((((....)))))....((((((((....(((((.(((((...(((((((..((.((((.......((
((....))))......)))).)).)))))))((((((((....(((((((............(((((((((...(((((.........(((.((((((...((...))...)))))).))).)))))...)))))).)))...((((....(((......))).))))......))))).))..))))))))....((((
((.(((....)))))))))........((((((((((((((.((((...............(((((((..(((((((((((((((((.....................))))))))))))))))).(((.(((.((((................((((....))))((((((((....((((((......))))))....
....((((............))))..))))))))..........)))).))).))).........(((.((.....)).)))..(((((...(((((((.................(((((.........((((.....((((.....((((((..(((((...........((((.............((((.......
...)))).....................................(((....)))))))...........)))))...))))))))))....)))).)))))........................((((((.(((....))).))))))(((((((....)))))))....((((((((...((((((..((((((....
.(((.....)))..))))))...))))))....)).))))))(((((((........((......))......))))))).(((..((((.......))))..)))..((.((((.....))))))..............(((((.((((...(((....)))..)))).))))).........................
...))))))).....)))))..))).))))...........((((..((((((((((........((((.....................)))).....)))))..........(((.....)))...)))))..)))).....................)))).)))................(((((....)))))..
......)))))))))))(((....(((((...)).)))....))).................)))))))))))))).))))......(((..(((((....))))).))).........................)))))).)))))...(((......)))..(((((....))))))))))))).....))))).)))
...........)))))..........((((.((......)).)))))))))).))))...)).)))))))).))))))).))).)))))))..))))..(((((.(((((((((........)))...................(((((..........(((((.......)))))........................
......(((.....)))......................(((..(((((......)))))..)))..((((((...))))))...)))))......))))))..)))))............((((....))))......

You can download the minimum free energy (MFE) structure in Vienna Format

II. Results for thermodynamic ensemble prediction

1. The free energy of the thermodynamic ensemble is [-368.26] kcal/mol.

2. The frequency of mfe structure in ensemble 4.72519e-27.

3. The ensemble diversity 421.25.

4. You may look at the dot plot containing the base pair probabilities [below]

Download Dot plot JPG EPS

III. Graphical output

1. A note on base-pairing probabilities:--

The structure below is colored by base-pairing probabilities. For unpaired regions the color denotes the probability of being unpaired. The reliability information of RNA secondary structure plot in the form of color annotation, Positional entropy ranging from red (low entropy, well-defined) via green to blue and violet (high entropy, ill-defined).

Download RNA structure JPG EPS

2. Here you find a mountain plot representation of the MFE structure, the thermodynamic ensemble of RNA structures, and the centroid structure. Additionally we present the positional entropy for each position. The resulting plot shows three curves, two mountain plots derived from the MFE structure (red) and the pairing probabilities (black) and a positional entropy curve (green). Well-defined regions are identified by low entropy.

Download mountain plot JPG EPS